ZFIN ID: ZDB-PUB-160324-7
Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy
Fu, Q., Martin, B.L., Matus, D.Q., Gao, L.
Date: 2016
Source: Nature communications   7: 11088 (Journal)
Registered Authors: Martin, Benjamin
Keywords: Biological sciences, Biotechnology, Cell biology
MeSH Terms:
  • Animals
  • Caenorhabditis elegans*
  • Embryo, Nonmammalian/anatomy & histology*
  • Imaging, Three-Dimensional/methods*
  • Microscopy/methods*
  • Zebrafish*
PubMed: 27004937 Full text @ Nat. Commun.
Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes SPIM live imaging performance by using a real-time adjustable tiling light sheet and creating a flexible compromise between spatial and temporal resolution. We demonstrate the 3D live imaging ability of TLS-SPIM by imaging cellular and subcellular behaviours in live C. elegans and zebrafish embryos, and show how TLS-SPIM can facilitate cell biology research in multicellular specimens by studying left-right symmetry breaking behaviour of C. elegans embryos.