ZFIN Image: Fu et al., 2016, Fig. 5

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Figure Caption

Fig. 5

3D imaging by TLS-SPIM with real-time light sheet optimization.(a) An ideal imaging method with both high spatial resolution and imaging speed acquires spatial and temporal information simultaneously. (b) A compromised solution acquires spatial information and temporal information separately by quick switching between a high spatial resolution, low imaging speed mode and a low spatial resolution, high imaging speed mode. (c,d) 3D volume renderings of two mesodermal cells in the tailbud of a ~15 hpf zebrafish embryo imaged in a low spatial resolution, high imaging speed mode and a high spatial resolution, low imaging speed mode. (e,f) Orthogonal views of the cells in c and d. (g) Ten continuous time points of the selected area in c imaged in the low spatial resolution, high imaging speed mode, showing the lamellipodia activities of the mesodermal cell. Scale bars, 5 µm (c-f), 2 µm (g).

Acknowledgments

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