FIGURE

Fig. 3

ID

ZDB-FIG-160317-19

Publication

Sempou et al., 2016 - Activation of zebrafish Src family kinases by the prion protein is an amyloid-β-sensitive signal that prevents the endocytosis and degradation of E-cadherin/β-catenin complexes in vivo

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Fig. 3

PrP-1 regulates AJs via Fyn and Yes. a. Localization of AJ components (immunofluorescence) in deep (top) and EVL cells (bottom) of 6 hpf PrP-1, Fyn/Yes morphants or PrP-1 morphants co-injected with Fyn/Yes mRNAs. Scale bars = 10 µM. See also Additional file 1: Figure S2. b. Reduction in Src/Yes (65 kDa) and Fyn (50 kDa) total levels in 6 hpf Fyn and Yes single or double knockdowns. c, d. Levels of AJ components in 6 hpf embryo lysates. Activated WT or CA Fyn- and Yes-EGFP were detected with an anti-phospho-Y416 Src antibody. E-cadherin arrowheads as in Fig. 1. WB = Western blot. Densitometric analysis of Western blot bands is expressed in arbitrary units (AU) in b, c and d; average values of three independent experiments ± SEM are shown; statistical significance was assessed using unpaired, two-tailed t-tests; ns = not significant (p > 0.05), * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001

Expression Data

Antibody:	Ab2-ctnnb
Fish:	WT WT + MO1-prnpb + MO2-prnpb WT + MO1-fyna,fynb + MO1-yes1
Knockdown Reagents:	MO1-fyna,fynb MO1-prnpb MO1-yes1 MO2-prnpb
Anatomical Terms:	DEL cell surface DEL cytosol DEL intracellular anatomical structure EVL cell surface whole organism
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-prnpb + MO2-prnpb WT + MO1-fyna,fynb + MO1-yes1
Knockdown Reagents:	MO1-fyna,fynb MO1-prnpb MO1-yes1 MO2-prnpb
Observed In:	DEL cytosol DEL intracellular anatomical structure whole organism
Stage:	Shield

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Neurodegener.