Fig. 5
Migration Characteristics of Stable-Bleb Cells In Vitro (A) Illustration of progenitor cells cultured under serum/LPA-free (I, II) or serum/LPA-containing (III, IV) conditions with (II, IV) or without (I, III) confinement. (A′) Table summarizing cell migration characteristics for different culture conditions. (B–D) N-Cadherin-GFP (B), Ezrin-YFP (C), and Integrinα5-Integrinβ1-BiFc-Venus (D) localization in polarized stable-bleb cells. (E) Representative tracks of migrating stable-bleb cells. (F) Mean-square displacement (MSD) analysis data (blue, n = 67) with fit to a persistent random walk model (gray) and compared to random (~t, orange) and directed migration (~t2, red). Black dashed line highlights random migration on timescales above the persistence time Pt ~10 min (persistence length H150 µm). (F′) Sketch of MSD time point analysis. Arrows denote variations in the direction of movement affecting persistence of cell movement. (G) Average instantaneous stable-bleb cell migration speeds in confinement between glass-agarose or glass-glass assays for varying substrate coatings as indicated. (H–J) Localization of Paxillin-GFP for stable-bleb cells on BSA- (H), PEG- (I), and Fibronectin-coated (J) glass substrates in confinement. (K) Paxillin-GFP localization for mesodermal progenitors cultured on 2D planar substrates coated with Fibronectin. n denotes number of cells. White dashed lines in (H)–(K) outline cell contact areas. All scale bars represent 10 µm. See also Extended Experimental Procedures. |
Reprinted from Cell, 160, Ruprecht, V., Wieser, S., Callan-Jones, A., Smutny, M., Morita, H., Sako, K., Barone, V., Ritsch-Marte, M., Sixt, M., Voituriez, R., Heisenberg, C.P., Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility, 673-685, Copyright (2015) with permission from Elsevier. Full text @ Cell