PUBLICATION
Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility
- Authors
- Ruprecht, V., Wieser, S., Callan-Jones, A., Smutny, M., Morita, H., Sako, K., Barone, V., Ritsch-Marte, M., Sixt, M., Voituriez, R., Heisenberg, C.P.
- ID
- ZDB-PUB-150214-3
- Date
- 2015
- Source
- Cell 160: 673-685 (Journal)
- Registered Authors
- Barone, Vanessa, Heisenberg, Carl-Philipp, Morita, Hitoshi, Ruprecht, Verena, Sako, Keisuke, Smutny, Michael
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Adhesion
- Cell Movement*
- Cell Polarity
- Embryo, Nonmammalian/cytology*
- Gastrula/cytology*
- Stem Cells/cytology*
- Zebrafish/embryology*
- PubMed
- 25679761 Full text @ Cell
Citation
Ruprecht, V., Wieser, S., Callan-Jones, A., Smutny, M., Morita, H., Sako, K., Barone, V., Ritsch-Marte, M., Sixt, M., Voituriez, R., Heisenberg, C.P. (2015) Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility. Cell. 160:673-685.
Abstract
3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping