ZFIN ID: ZDB-FIG-150326-35
Dyer et al., 2014 - Specification of sensory neurons occurs through diverse developmental programs functioning in the brain and spinal cord. Developmental dynamics : an official publication of the American Association of Anatomists   243(11):1429-39 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 5

Ngn1 function is required for RB neuron development, but not for MTN development. A–C: Dorsal views of the brains of 24-hpf Tg[elavl3:egfp] embryos processed by fluorescent in situ for ngn1 and labelled with anti-GFP. Orthogonal views at the level of MTN neurons show there is no co-localisation of ngn1 and EGFP in nTPC (asterisk) or MTN neurons (arrowheads, C). D–F: A comparison of huc/elavl3 expression to GFP in the brain of a 24-hpf Tg[-8.4neurog1:GFP] transgenic larvae does not reveal co-localisation in nTPC or MTN neurons, although some epiphyseal neurons (ep) do show co-expression. Uninjected (G,I,K) or embryos injected with ngn1 morpholino (H,J,L), then fixed at 24 hpf. Lateral views of the spinal cord reveal that isl1 expression in absent from RB neurons in ngn1 morphants (G,H). A dorsal view of the spinal cord labelled with zn-12 reveals a loss of RB neurons (arrows) and reticulospinal tract axons (I,J). Dorsal views of the head reveal that isl1 expressing MTN and nTPC neurons appear to be unaffected in ngn1 morphants (K,L). M: Quantification of RB, MTN,and nTPC neurons expressing drg11 reveals that ngn1 loss of function results in a dramatic reduction of RB neurons (**P<0.01), but does not result in a significant change (NS) of MTN or nTPC neuron number.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP knu3Tg standard conditions Prim-5 mesencephalic nucleus of trigeminal nerve IHC
Prim-5 nucleus of the caudal commissure IHC
GFP sb1Tg standard conditions Prim-5 epiphysis IHC
Prim-5 midbrain IHC
isl1a WT control Prim-5 mesencephalic nucleus of trigeminal nerve ISH
Prim-5 nucleus of the caudal commissure ISH
Prim-5 Rohon-Beard neuron ISH
WT + MO4-neurog1 standard conditions Prim-5 mesencephalic nucleus of trigeminal nerve ISH
Prim-5 nucleus of the caudal commissure ISH
Prim-5 Not Detected Rohon-Beard neuron ISH
neurog1 knu3Tg standard conditions Prim-5 midbrain ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
zn-12 IHC WT control Prim-5 Rohon-Beard neuron
IHC Prim-5 Rohon-Beard neuron axon
IHC WT + MO4-neurog1 standard conditions Prim-5 Not Detected Rohon-Beard neuron
IHC Prim-5 Not Detected Rohon-Beard neuron axon
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO4-neurog1 standard conditions Prim-5 Rohon-Beard neuron decreased amount, abnormal
Acknowledgments:
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