PUBLICATION

Specification of sensory neurons occurs through diverse developmental programs functioning in the brain and spinal cord

Authors
Dyer, C., Linker, C., Graham, A., Knight, R.
ID
ZDB-PUB-140903-7
Date
2014
Source
Developmental dynamics : an official publication of the American Association of Anatomists   243(11): 1429-39 (Journal)
Registered Authors
Knight, Robert
Keywords
Mesencephalic Trigeminal Nucleus, Notch, Rohon-Beard, neural crest, proprioception
MeSH Terms
  • Animals
  • Cell Differentiation/physiology
  • Central Nervous System/cytology*
  • Central Nervous System/embryology*
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental/physiology*
  • Image Processing, Computer-Assisted
  • Immunohistochemistry
  • In Situ Hybridization
  • Microscopy, Confocal
  • Nerve Tissue Proteins/metabolism*
  • Receptors, Notch/metabolism
  • Sensory Receptor Cells/metabolism
  • Sensory Receptor Cells/physiology*
  • Signal Transduction/physiology
  • Tegmentum Mesencephali/cytology
  • Transcription Factors/metabolism*
  • Zebrafish/embryology*
PubMed
25179866 Full text @ Dev. Dyn.
Abstract
Background Vertebrates possess two populations of sensory neurons located within the central nervous system: Rohon-Beard (RB) and mesencephalic trigeminal nucleus (MTN) neurons. RB neurons are transient spinal cord neurons whilst MTN neurons are the proprioceptive cells that innervate the jaw muscles. It has been suggested that MTN and RB neurons share similarities and may have a common developmental program, but it is unclear how similar or different their development is. Results We have dissected RB and MTN neuronal specification in zebrafish. We find that RB and MTN neurons express a core set of genes indicative of sensory neurons, but find these are also expressed by adjacent diencephalic neurons. Unlike RB neurons, our evidence argues against a role for the neural crest during MTN development. We additionally find that neurogenin1 function is dispensable for MTN differentiation, unlike RB cells and all other sensory neurons. Finally, we demonstrate that, although Notch signalling is involved in RB development, it is not involved in the generation of MTN cells. Conclusions Our work reveals fundamental differences between the development of MTN and RB neurons and suggests that these populations are non-homologous and thus have distinct developmental and, probably, evolutionary origins.
Genes / Markers
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Human Disease / Model
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Antibodies
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Mapping