PUBLICATION

Specification of sensory neurons occurs through diverse developmental programs functioning in the brain and spinal cord

Authors
Dyer, C., Linker, C., Graham, A., Knight, R.
ID
ZDB-PUB-140903-7
Date
2014
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   243(11): 1429-39 (Journal)
Registered Authors
Knight, Robert
Keywords
Mesencephalic Trigeminal Nucleus, Notch, Rohon-Beard, neural crest, proprioception
MeSH Terms
  • In Situ Hybridization
  • Cell Differentiation/physiology
  • Immunohistochemistry
  • Nerve Tissue Proteins/metabolism*
  • Animals
  • Central Nervous System/cytology*
  • Central Nervous System/embryology*
  • Tegmentum Mesencephali/cytology
  • Transcription Factors/metabolism*
  • Zebrafish/embryology*
  • Gene Expression Profiling
  • Signal Transduction/physiology
  • Sensory Receptor Cells/metabolism
  • Sensory Receptor Cells/physiology*
  • Receptors, Notch/metabolism
  • Gene Expression Regulation, Developmental/physiology*
  • Image Processing, Computer-Assisted
  • Microscopy, Confocal
(all 18)
PubMed
25179866 Full text @ Dev. Dyn.
Abstract
Background Vertebrates possess two populations of sensory neurons located within the central nervous system: Rohon-Beard (RB) and mesencephalic trigeminal nucleus (MTN) neurons. RB neurons are transient spinal cord neurons whilst MTN neurons are the proprioceptive cells that innervate the jaw muscles. It has been suggested that MTN and RB neurons share similarities and may have a common developmental program, but it is unclear how similar or different their development is. Results We have dissected RB and MTN neuronal specification in zebrafish. We find that RB and MTN neurons express a core set of genes indicative of sensory neurons, but find these are also expressed by adjacent diencephalic neurons. Unlike RB neurons, our evidence argues against a role for the neural crest during MTN development. We additionally find that neurogenin1 function is dispensable for MTN differentiation, unlike RB cells and all other sensory neurons. Finally, we demonstrate that, although Notch signalling is involved in RB development, it is not involved in the generation of MTN cells. Conclusions Our work reveals fundamental differences between the development of MTN and RB neurons and suggests that these populations are non-homologous and thus have distinct developmental and, probably, evolutionary origins.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WTchemical treatment: DAPTPrim-5
WTchemical treatment: DAPTPrim-5
WTchemical treatment: DAPTPrim-5
WTchemical treatment: DAPTPrim-5
WT + MO4-neurog1standard conditionsPrim-5
sox10baz1/baz1standard conditionsPrim-5
1 - 6 of 6
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
baz1
    		Point Mutation
    knu3TgTransgenic Insertion
      sb1TgTransgenic Insertion
        zf15TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Fish
          Antibodies
          Orthology
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          GFPEFGGFP
          1 - 2 of 2
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          Mapping
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          Citation
          Dyer, C., Linker, C., Graham, A., Knight, R. (2014) Specification of sensory neurons occurs through diverse developmental programs functioning in the brain and spinal cord. Developmental Dynamics : an official publication of the American Association of Anatomists. 243(11):1429-39.