ZFIN ID: ZDB-FIG-140807-32
Araya et al., 2014 - Mesoderm is required for coordinated cell movements within zebrafish neural plate in vivo. Neural Development   9(1):9 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Fig. 1 MZoep mutants have aberrant neural tube organization. (A) Projection of confocal z-series showing dorsal view of brain and anterior spinal cord. The midline ventricle is lined by ZO-1 expression (green) and the basal regions of the neuroepithelium are lined by the GFAP expression (red). (B) Projection of confocal z-series showing dorsal view of brain and anterior spinal cord from MZoep mutant. Both apical ZO-1 and basal GFAP expression show extensive disruption to neural tube morphology in brain regions (arrowheads) but appear relatively normal in anterior spinal cord (arrow). (C) Projection of confocal z-series showing dorsal view of brain and anterior spinal cord from embryo treated with the Nodal inhibitor SB-431542. (D,E) Transverse sections show the normal single midline domain of ZO-1 appears discontinuous and more randomly oriented in MZoep embryo. (F,G) The basal marker GFAP is expressed in ectopic foci deep from the surface of the MZoep neural primordium. (H,I,J,K) Neurons labeled with tg(HUC-GFP) (green) and their axons labeled with Ac-tub (red) are present but disorganized in the Nodal-defective embryo brains. (L) By 28 hpf ectopic ventricles (arrowed) have opened up in the MZoep brains. Ac-tub, anti-acetylated tubulin antibody; GFAP, glial fibrillary acidic protein; hpf, hours post fertilization; MZoep, maternal-zygotic one-eyed pinhead; ZO-1, zonula occludens 1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg standard conditions Prim-5 neuron IHC
knu3Tg chemical treatment: pharmaceutical Prim-5 neuron IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tjp1 IHC WT standard conditions Prim-5 ventricular system apical surface
IHC WT chemical treatment: pharmaceutical Prim-5 neural tube
IHC tdgf1tz257/tz257 standard conditions Prim-5 neural tube
Ab2-gfap IHC WT standard conditions Prim-5 neuroepithelial cell basal region
IHC WT chemical treatment: pharmaceutical Prim-5 neural tube
IHC tdgf1tz257/tz257 standard conditions Prim-5 neural tube
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: pharmaceutical Prim-5 neural tube morphology, abnormal
knu3Tg chemical treatment: pharmaceutical Prim-5 neural tube morphology, abnormal
tdgf1tz257/tz257 standard conditions Prim-5 neural tube morphology, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.