ZFIN ID: ZDB-FIG-140506-50
Pavlou et al., 2014 - Pleiotropic effects of Sox2 during the development of the zebrafish epithalamus. PLoS One   9(1):e87546 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 5

Sox2 and Notch have synergistic effect on neurogenesis and complementary effects on cell-fate determination.

(A–D) The number of pineal neurons (Isl1-positive cells) is increased in sox2 morphants (B) and in DAPT-treated embryos (C), when compared to DMSO-treated controls (A). A synergistic effect is observed when both sox2 and Notch are knocked down (D). (E–H) Tg(aanat2:GFP) drives GFP expression in the pineal PhRs in DMSO-treated controls (E). The number of PhRs is increased in sox2 morphants (F), but remains unaffected in DAPT-treated embryos (G). The knockdown of both sox2 and Notch results in an increased number of GFP-positive cells (H), comparable to the one observed in sox2 morphants. (I–L) Tg(elavl3:GFP) expresses GFP in PNs. There is no difference in the number of GFP-positive in sox2 morphants (I) when compared to DMSO-treated controls (I). The knockdown of Notch alone (K) or simultaneously with sox2 (L) results in similar increase in GFP expression. (M–O) Average number of Isl1-positive cells (M), PhRs (N) and PNs (O) in untreated controls, DMSO-treated controls, untreated sox2 morphants, DMSO-treated sox2 morphants, DAPT-treated embryos and DAPT-treated sox2 morphants. Confocal maximum projections of 28 hpf embryos, scale bars = 25 μm, error bars represent ± standard error, * = p-value <0.05 (MWU test), ** = p-value <0.001 (MWU test). See also Figure S5–S7 and Movie S1–S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg control Prim-5 pineal complex neuron IHC
knu3Tg chemical treatment: DAPT Prim-5 pineal complex neuron IHC
knu3Tg + MO6-sox2 chemical treatment: DAPT Prim-5 pineal complex neuron IHC
knu3Tg + MO6-sox2 standard conditions Prim-5 pineal complex neuron IHC
y8Tg control Prim-5 pineal complex photoreceptor cell IHC
y8Tg chemical treatment: DAPT Prim-5 pineal complex photoreceptor cell IHC
y8Tg + MO6-sox2 chemical treatment: DAPT Prim-5 pineal complex photoreceptor cell IHC
y8Tg + MO6-sox2 standard conditions Prim-5 pineal complex photoreceptor cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-isl IHC WT control Prim-5 pineal complex neuron
IHC WT chemical treatment: DAPT Prim-5 pineal complex neuron
IHC WT + MO6-sox2 chemical treatment: DAPT Prim-5 pineal complex neuron
IHC WT + MO6-sox2 standard conditions Prim-5 pineal complex neuron
Phenotype Details
Fish Conditions Stage Phenotype
y8Tg + MO6-sox2 chemical treatment: DAPT Prim-5 pineal complex photoreceptor cell increased amount, abnormal
y8Tg + MO6-sox2 standard conditions Prim-5 pineal complex photoreceptor cell increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One