ZFIN ID: ZDB-FIG-140506-50 |
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Anatomical Terms: | |
Stage: | Prim-5 |
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Observed In: | |
Stage: | Prim-5 |
Fig. 5 Sox2 and Notch have synergistic effect on neurogenesis and complementary effects on cell-fate determination. (A–D) The number of pineal neurons (Isl1-positive cells) is increased in sox2 morphants (B) and in DAPT-treated embryos (C), when compared to DMSO-treated controls (A). A synergistic effect is observed when both sox2 and Notch are knocked down (D). (E–H) Tg(aanat2:GFP) drives GFP expression in the pineal PhRs in DMSO-treated controls (E). The number of PhRs is increased in sox2 morphants (F), but remains unaffected in DAPT-treated embryos (G). The knockdown of both sox2 and Notch results in an increased number of GFP-positive cells (H), comparable to the one observed in sox2 morphants. (I–L) Tg(elavl3:GFP) expresses GFP in PNs. There is no difference in the number of GFP-positive in sox2 morphants (I) when compared to DMSO-treated controls (I). The knockdown of Notch alone (K) or simultaneously with sox2 (L) results in similar increase in GFP expression. (M–O) Average number of Isl1-positive cells (M), PhRs (N) and PNs (O) in untreated controls, DMSO-treated controls, untreated sox2 morphants, DMSO-treated sox2 morphants, DAPT-treated embryos and DAPT-treated sox2 morphants. Confocal maximum projections of 28 hpf embryos, scale bars = 25 μm, error bars represent ± standard error, * = p-value <0.05 (MWU test), ** = p-value <0.001 (MWU test). See also Figure S5–S7 and Movie S1–S2. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
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EGFP | knu3Tg | control | Prim-5 | pineal complex neuron | IHC | |
knu3Tg | chemical treatment: DAPT | Prim-5 | pineal complex neuron | IHC | ||
knu3Tg + MO6-sox2 | chemical treatment: DAPT | Prim-5 | pineal complex neuron | IHC | ||
knu3Tg + MO6-sox2 | standard conditions | Prim-5 | pineal complex neuron | IHC | ||
y8Tg | control | Prim-5 | pineal complex photoreceptor cell | IHC | ||
y8Tg | chemical treatment: DAPT | Prim-5 | pineal complex photoreceptor cell | IHC | ||
y8Tg + MO6-sox2 | chemical treatment: DAPT | Prim-5 | pineal complex photoreceptor cell | IHC | ||
y8Tg + MO6-sox2 | standard conditions | Prim-5 | pineal complex photoreceptor cell | IHC |
Antibody | Assay | Fish | Conditions | Stage | Anatomy |
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Ab2-isl | IHC | WT | control | Prim-5 | pineal complex neuron |
IHC | WT | chemical treatment: DAPT | Prim-5 | pineal complex neuron | |
IHC | WT + MO6-sox2 | chemical treatment: DAPT | Prim-5 | pineal complex neuron | |
IHC | WT + MO6-sox2 | standard conditions | Prim-5 | pineal complex neuron |
Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One |
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