| ZFIN ID: ZDB-FIG-140506-49 |
| Gene: | |
|---|---|
| Antibody: | |
| Fish: | |
| Knockdown Reagent: | |
| Anatomical Terms: | |
| Stage: | Prim-5 |
| Fish: | |
|---|---|
| Knockdown Reagent: | |
| Observed In: | |
| Stage: | Prim-5 |
Fig. 4
C) Tg(aanat2:GFP) drives GFP expression in the pineal PhRs. (B) Isl1 labels the pineal cells. (D–F) Knockdown of sox2 results in increased number of PhRs. (G) The average number of PhRs in control (purple bar) and sox2 morphants (orange bar). (H–J) Tg(elavl3:GFP) drives GFP expression specifically in the PNs. (K–M) The knockdown of sox2 does not affect the number of PNs. (N) The average number of PNs in controls (purple bar) and sox2 morphants (orange bar) does not significantly differ. Confocal maximum projections of 28 hpf embryos, scale bars = 25 μm, error bars represent ± standard error, ** = p-value <0.001 (MWU test). See also Figure S4. |
| Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
|---|---|---|---|---|---|---|
| EGFP | knu3Tg | control | Prim-5 | pineal complex neuron | IHC | |
| knu3Tg + MO6-sox2 | standard conditions | Prim-5 | pineal complex neuron | IHC | ||
| y8Tg | control | Prim-5 | pineal complex photoreceptor cell | IHC | ||
| y8Tg + MO6-sox2 | standard conditions | Prim-5 | pineal complex photoreceptor cell | IHC |
| Antibody | Assay | Fish | Conditions | Stage | Anatomy |
|---|---|---|---|---|---|
| Ab2-isl | IHC | knu3Tg | control | Prim-5 | pineal complex neuron |
| IHC | knu3Tg + MO6-sox2 | standard conditions | Prim-5 | pineal complex neuron | |
| IHC | y8Tg | control | Prim-5 | pineal complex neuron | |
| IHC | y8Tg + MO6-sox2 | standard conditions | Prim-5 | pineal complex neuron |
| Fish | Conditions | Stage | Phenotype |
|---|---|---|---|
| y8Tg + MO6-sox2 | standard conditions | Prim-5 | pineal complex photoreceptor cell increased amount, abnormal |
| Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One |
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