ZFIN ID: ZDB-FIG-140506-49
Pavlou et al., 2014 - Pleiotropic effects of Sox2 during the development of the zebrafish epithalamus. PLoS One   9(1):e87546 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 4

C) Tg(aanat2:GFP) drives GFP expression in the pineal PhRs. (B) Isl1 labels the pineal cells. (D–F) Knockdown of sox2 results in increased number of PhRs. (G) The average number of PhRs in control (purple bar) and sox2 morphants (orange bar). (H–J) Tg(elavl3:GFP) drives GFP expression specifically in the PNs. (K–M) The knockdown of sox2 does not affect the number of PNs. (N) The average number of PNs in controls (purple bar) and sox2 morphants (orange bar) does not significantly differ. Confocal maximum projections of 28 hpf embryos, scale bars = 25 μm, error bars represent ± standard error, ** = p-value <0.001 (MWU test). See also Figure S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg control Prim-5 pineal complex neuron IHC
knu3Tg + MO6-sox2 standard conditions Prim-5 pineal complex neuron IHC
y8Tg control Prim-5 pineal complex photoreceptor cell IHC
y8Tg + MO6-sox2 standard conditions Prim-5 pineal complex photoreceptor cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-isl IHC knu3Tg control Prim-5 pineal complex neuron
IHC knu3Tg + MO6-sox2 standard conditions Prim-5 pineal complex neuron
IHC y8Tg control Prim-5 pineal complex neuron
IHC y8Tg + MO6-sox2 standard conditions Prim-5 pineal complex neuron
Phenotype Details
Fish Conditions Stage Phenotype
y8Tg + MO6-sox2 standard conditions Prim-5 pineal complex photoreceptor cell increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One