ZFIN ID: ZDB-FIG-140430-9
Al Oustah et al., 2014 - Dynamics of Sonic hedgehog signaling in the ventral spinal cord are controlled by intrinsic changes in source cells requiring Sulfatase 1. Development (Cambridge, England)   141(6):1392-1403 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 1

Sulf1 depletion impairs OPC development in zebrafish. A-H show side views of whole embryos at the level of the trunk spinal cord; anterior to the left and dorsal to the top. J-L show transverse spinal cord sections, dorsal to the top. (A-C) Detection (A,B) and quantification (C) of dorsal OPCs (arrows) at 48 hpf in Tg(olig2:GFP) embryos injected with ctrlMO (A) and sulf1MO (B). (D-F) Detection (D,E) and quantification (F) of dorsal OPCs (arrows) at 72 hpf in Tg(nkx2.2a:mEGFP;olig2:dsRed2) larvae injected with ctrlMO (D) and sulf1MO (E). (G,H,J-L) Expression of mbpa at 72 hpf in larvae injected with sulf1 mismatch MO (G) and sulf1MO (H) and in wild-type (J), sulf1sa199+/- (K) and sulf1sa199-/- (L) larvae. (I,M) Quantification of mbpa+ cells in transverse sections of morphants (I) and sulf1sa199+/- incross progeny (M). Results are presented as mean number of cells ± s.e.m. (**P<0.01, ***P<0.001, ****P<0.0001; ns, non-significant). Brackets indicate position of the dorsal spinal cord. Scale bars: 100 μm (A-H), 50 μm (J-L).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 vu17Tg; vu19Tg control Protruding-mouth spinal cord oligodendrocyte IFL
vu17Tg; vu19Tg + MO5-sulf1 standard conditions Protruding-mouth spinal cord oligodendrocyte IFL
EGFP vu12Tg control Long-pec spinal cord oligodendrocyte IFL
vu12Tg + MO5-sulf1 standard conditions Long-pec spinal cord oligodendrocyte IFL
vu17Tg; vu19Tg control Protruding-mouth spinal cord oligodendrocyte IFL
vu17Tg; vu19Tg + MO5-sulf1 standard conditions Protruding-mouth spinal cord oligodendrocyte IFL
mbpa WT control Protruding-mouth spinal cord oligodendrocyte ISH
WT standard conditions Protruding-mouth spinal cord oligodendrocyte ISH
sulf1sa199/+ control Protruding-mouth spinal cord oligodendrocyte ISH
sulf1sa199/sa199 control Protruding-mouth spinal cord oligodendrocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sulf1sa199/+ control Protruding-mouth spinal cord has fewer parts of type oligodendrocyte, abnormal
Protruding-mouth spinal cord oligodendrocyte cell differentiation decreased occurrence, abnormal
sulf1sa199/sa199 control Protruding-mouth spinal cord has fewer parts of type oligodendrocyte, abnormal
Protruding-mouth spinal cord oligodendrocyte cell differentiation decreased occurrence, abnormal
vu12Tg + MO5-sulf1 standard conditions Long-pec spinal cord has fewer parts of type oligodendrocyte, abnormal
Long-pec spinal cord oligodendrocyte cell differentiation decreased occurrence, abnormal
vu17Tg; vu19Tg + MO5-sulf1 standard conditions Protruding-mouth spinal cord has fewer parts of type oligodendrocyte, abnormal
Protruding-mouth spinal cord oligodendrocyte cell differentiation decreased occurrence, abnormal
Acknowledgments:
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