FIGURE

Fig. 5

ID
ZDB-FIG-140430-5
Publication
Gao et al., 2014 - Expression and functional characterization of Smyd1a in myofibril organization of skeletal muscles
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

The effect of smyd1a or smyd1b single or double knockdown on the Z-line organization in skeletal muscles.

Zebrafish embryos injected with smyd1b MO or smyd1a MO or both were fixed at 28, 48 and 72 hpf. Z-line organization was analyzed by immunostaining with anti-α-actinin antibody (EA-53), and followed by FTIC-labeled secondary antibody. The images represent side view of trunk muscles around segment 10. A–C. Lateral view of Z-line organization in skeletal muscle fibers of control-MO injected embryos at 28 (A), 48 (B) and 72 (C) hpf. D–F. Lateral view of Z-line organization in skeletal muscle fibers of smyd1a E8I8-MO injected embryos at 28 (D), 48 (E) and 72 (F) hpf. G–I. Lateral view of Z-line organization in skeletal muscle fibers of smyd1a E8I8-MO and E9I9-MO co-injected embryos at 28 (G), 48 (H) and 72 (I) hpf. J–L. Lateral view of Z-line organization in skeletal muscle fibers of smyd1b ATG-MO injected embryos at 28 (J), 48 (K) and 72 (L) hpf. M–O. Lateral view of Z-line organization in skeletal muscle fibers of smyd1a E8I8-MO and smyd1b ATG-MO co-injected embryos at 28 (M), 48 (N) and 72 (O) hpf. Scale bars: 20 μm in A–C.

Expression Data
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Prim-5 to Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One