FIGURE

Fig. 2

ID
ZDB-FIG-140403-2
Publication
Cox et al., 2014 - S-Nitrosothiol Signaling Regulates Liver Development and Improves Outcome following Toxic Liver Injury
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Fig. 2

Constitutive NOS Activity Is Required for Optimal Liver Development

(A) Effect of morpholino-mediated knockdown of nos1 and nos2 gene expression on liver formation as assessed by in situ hybridization for lfabp at 72 hpf. Translation blocking ATG morpholinos against nos 1 and 2 were injected at a concentration of 50 μM. Representative photomicrographs were taken at 10× magnification.

(B) Effect of nos knockdown on liver size in Tg(lfabp:GFP) embryos. Representative fluorescent photomicrographs were taken at 10× magnification.

(C) Effect of nos knockdown on hepatic progenitor cells as assessed by in situ hybridization for foxA3 at 48 hpf. Representative photomicrographs were taken at 10× magnification.

(D) Phenotype analysis of liver size (lfabp) in morphants at 72 hpf (n > 50 embryos per treatment).

(E) Effect of nos knockdown on the percentage of hepatocytes specified during liver formation. Tg(lfabp:GFP) morphants were dissociated, and the percentage of GFP positive hepatocytes was analyzed by FACS.

n = 8; ANOVA, p = 0.033 in comparison to control. Results in (E) show mean ± SEM.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Long-pec to Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Phenotype Detail
Acknowledgments
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