ZFIN ID: ZDB-FIG-130816-15
Minchin et al., 2013 - Oesophageal and sternohyal muscle fibres are novel Pax3-dependent migratory somite derivatives essential for ingestion. Development (Cambridge, England)   140(14):2972-2984 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 4 OSM is Myod dependent in zebrafish. In situ hybridisation for myog (A,C,D) or mylz2 (A), and immunodetection of MyHC (B) and Myh11 (B). Dorsal view wholemounts are oriented anterior towards the left (A, upper panels; C,D). Lateral view wholemounts are oriented anterior towards the left and dorsal towards the top (A, lower panels; B). (A) At 72 hpf, myog mRNA expression was indistinguishable from that in wild-type siblings in OSM of myf5hu2022 mutants (red arrowheads). PHM, PFM and pharynx muscle were also unchanged (white arrowheads, green arrowheads and asterisk, respectively). Myosin mRNA (mylz2) at 120 hpf was unaltered in myf5hu2022 mutants, suggesting OSM terminal differentiation occurs normally after reduction of Myf5 (red arrowheads). (B) OSM and smooth muscle differentiation was unaffected in myf5hu2022 mutants (red arrowheads and white arrows, respectively). In myodfh261 mutants, MyHC within OSM was absent, but SHM and PHM differentiated well (yellow and white arrowheads, respectively), as did oesophageal smooth muscle (white arrows). Double myf5hu2022;myodfh261 mutants had no striated skeletal muscle, but cardiac striated muscle (h) was readily observed, as was smooth muscle in the gastrointestinal tract and bulbus arteriosus (bu). (C,D) myog mRNA reduction in the oesophageal region of myodfh261 mutants (C) and myod morphants (D). Asterisk indicates pharyngeal muscle. Arrowheads indicate OSM (red), SHM (yellow), pectoral fin muscle (green) and PHM (white). White arrow indicates oesophageal smooth muscle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mylpfa WT control Day 5 esophageal sphincter ISH
Day 5 esophagus skeletal muscle cell ISH
myf5hu2022/hu2022 standard conditions Day 5 esophageal sphincter ISH
Day 5 esophagus skeletal muscle cell ISH
myog WT control Protruding-mouth esophagus skeletal muscle cell ISH
Protruding-mouth pectoral fin musculature ISH
WT + MO1-myod1 standard conditions Protruding-mouth esophagus skeletal muscle cell ISH
myf5hu2022/hu2022 standard conditions Protruding-mouth esophagus skeletal muscle cell ISH
Protruding-mouth pectoral fin musculature ISH
myod1fh261/fh261 standard conditions Protruding-mouth Not Detected esophagus skeletal muscle cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT control Day 5 sternohyoid
IHC myf5hu2022/hu2022 standard conditions Day 5 sternohyoid
IHC myf5hu2022/hu2022; myod1fh261/fh261 standard conditions Day 5 sternohyoid
IHC myod1fh261/fh261 standard conditions Day 5 sternohyoid
Ab1-myh11 IHC WT control Day 5 bulbus arteriosus
IHC Day 5 gut smooth muscle
IHC myf5hu2022/hu2022 standard conditions Day 5 bulbus arteriosus
IHC Day 5 gut smooth muscle
IHC myf5hu2022/hu2022; myod1fh261/fh261 standard conditions Day 5 bulbus arteriosus
IHC Day 5 gut smooth muscle
IHC myod1fh261/fh261 standard conditions Day 5 bulbus arteriosus
IHC Day 5 gut smooth muscle
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-myod1 standard conditions Protruding-mouth esophagus skeletal muscle cell poorly differentiated, abnormal
Protruding-mouth head muscle poorly differentiated, abnormal
myf5hu2022/hu2022 standard conditions Day 5 enteric musculature differentiated, normal
Day 5 esophagus skeletal muscle cell differentiated, normal
Day 5 myocardium differentiated, normal
Day 5 smooth muscle cell differentiation process quality, normal
myf5hu2022/hu2022; myod1fh261/fh261 standard conditions Day 5 bulbus arteriosus smooth muscle cell differentiated, normal
Day 5 enteric musculature differentiated, normal
Day 5 intestine smooth muscle cell differentiated, normal
Day 5 myocardium differentiated, normal
Day 5 skeletal muscle cell differentiation decreased process quality, abnormal
Day 5 smooth muscle cell differentiation process quality, normal
Day 5 whole organism lacks all parts of type skeletal muscle, abnormal
myod1fh261/fh261 standard conditions Protruding-mouth esophagus skeletal muscle cell poorly differentiated, abnormal
Protruding-mouth head muscle poorly differentiated, abnormal
Day 5 enteric musculature differentiated, normal
Day 5 esophagus skeletal muscle cell poorly differentiated, abnormal
Day 5 esophagus smooth muscle cell differentiated, normal
Day 5 head muscle poorly differentiated, abnormal
Day 5 myocardium differentiated, normal
Day 5 smooth muscle cell differentiation process quality, normal
Day 5 sternohyoid differentiated, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development