ZFIN ID: ZDB-FIG-130814-12
Reimer et al., 2013 - Dopamine from the Brain Promotes Spinal Motor Neuron Generation during Development and Adult Regeneration. Developmental Cell   25(5):478-491 Full text @ Dev. Cell
ADDITIONAL FIGURES
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Stage: Adult

Fig. 7

Dopamine Promotes Motor Neuron Regeneration in the Adult Spinal Cord Spinal cross-sections are shown at 14 dpl; central canal is indicated by dots or asterisks. (A) TH1+ axons and d4a mRNA expression are detectable mainly rostral to a spinal lesion site. (B) Higher magnification shows apposition (arrow) of TH1+ axons with radial processes of olig2:GFP+ motor neuron progenitor cells. (C) Ablation of dopaminergic axons with 6-OHDA reduces (p < 0.05) numbers of newly generated HB9:GFP+ motor neurons only rostral to a spinal lesion site. (D) NPA injections significantly (p < 0.05, one sided) increase the number of HB9+ motor neurons only caudal to a spinal lesion site. (E) NPA injections increase d4a (left) and patched2 (right) expression only caudal to the lesion site. Example gels and quantitative densitometric analyses are shown (p < 0.05, p < 0.01; Krukall-Wallis with Dunn’s posttest). (F) Schematic summary of dopamine signaling in relation to motor neuron regeneration at 14 dpl. Error bars represent SEM. Scale bars, 40 μm (A, left), 20 μm (A, right), 5 μm (B), and 15 µm (C and D). See also Figure S6.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
drd4a WT control Adult spinal cord ISH
WT transection: spinal cord Adult Not Detected spinal cord ISH
EGFP zf532Tg control Adult neuronal stem cell IFL
zf532Tg transection: spinal cord Adult neuronal stem cell IFL
GFP ml2Tg control Adult spinal cord motor neuron IFL
ml2Tg chemical treatment: drug Adult spinal cord motor neuron IFL
ml2Tg transection: spinal cord, chemical treatment: drug Adult spinal cord motor neuron IFL
ml2Tg transection: spinal cord Adult spinal cord motor neuron IFL
th Ab3-th WT control Adult dopaminergic neuron axon IHC
Ab3-th WT transection: spinal cord Adult Not Detected spinal cord IHC
Ab3-th zf532Tg control Adult dopaminergic neuron axon IHC
Ab3-th zf532Tg transection: spinal cord Adult dopaminergic neuron axon IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mnx1 IHC WT control Adult spinal cord motor neuron
IHC WT chemical treatment: pharmaceutical Adult spinal cord motor neuron
IHC WT transection: spinal cord, chemical treatment: pharmaceutical Adult spinal cord motor neuron
IHC WT transection: spinal cord Adult spinal cord motor neuron
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Cell, 25(5), Reimer, M.M., Norris, A., Ohnmacht, J., Patani, R., Zhong, Z., Dias, T.B., Kuscha, V., Scott, A.L., Chen, Y.C., Rozov, S., Frazer, S.L., Wyatt, C., Higashijima, S., Patton, E.E., Panula, P., Chandran, S., Becker, T., and Becker, C.G., Dopamine from the Brain Promotes Spinal Motor Neuron Generation during Development and Adult Regeneration, 478-491, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell