ZFIN ID: ZDB-FIG-130625-27
Nord et al., 2013 - Six1 regulates proliferation of Pax7-positive muscle progenitors in zebrafish. Journal of Cell Science   126(Pt 8):1868-80 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 4 six1b expression in Pax7+ cells is independent of MyoD and Myf5. (A) Cross-section of uninjected control embryos and myoD/myf5 morphant embryos at 24hpf showing mRNA expression of six1b (red) together with slow muscle marker F59 (green) or fast muscle marker F310 (green) and of Pax7 alone. six1b expression in the Pax7+ domain remains in MyoD/Myf5 double morphants. (B) Cross-section of control and myoD/myf5 morphant six1b:GFP embryos at 24hpf showing expression of Pax7 (red) together with six1b:GFP (green). six1b:GFP and Pax7 expression domains coincide in the myoD/myf5 morphants. Cross-sections were made at somite number 10. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP umu1Tg standard conditions Prim-5 myoblast IHC
umu1Tg + MO1-myod1 + MO3-myf5 standard conditions Prim-5 myoblast IHC
six1b WT standard conditions Prim-5 somite ISH
WT + MO1-myod1 + MO3-myf5 standard conditions Prim-5 somite ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC WT standard conditions Prim-5 slow muscle cell
IHC WT + MO1-myod1 + MO3-myf5 standard conditions Prim-5 slow muscle cell
Ab-F310 IHC WT standard conditions Prim-5 fast muscle cell
IHC WT + MO1-myod1 + MO3-myf5 standard conditions Prim-5 fast muscle cell
Ab-Pax7 IHC WT standard conditions Prim-5 myoblast
IHC WT + MO1-myod1 + MO3-myf5 standard conditions Prim-5 myoblast
IHC umu1Tg standard conditions Prim-5 myoblast
IHC umu1Tg + MO1-myod1 + MO3-myf5 standard conditions Prim-5 myoblast
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-myod1 + MO3-myf5 standard conditions Prim-5 fast muscle cell muscle cell development decreased process quality, abnormal
Prim-5 slow muscle cell muscle cell development decreased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.