FIGURE

Fig. 5

ID

ZDB-FIG-130401-25

Publication

Kondrychyn et al., 2013 - Stretching morphogenesis of the roof plate and formation of the central canal

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Fig. 5

Growth of the RP processes correlates with a shift of glyt1 expression along D–V axis.

(A-D), Double whole mount in situ hybridization (glyt1, dark purple) and immunostaining (GFP, green). glyt1 is expressed in the midline glial cells except RP; GFP is expressed in the RP cells. (E-J), Whole mount in situ hybridization (glyt1, dark purple) and immunostaining (β-catenin, red), the transverse sections of the spinal cord. The arrow shows an approximate position of attachment of the RP process to the apical surface of central canal. Confocal images of the spinal cord of pard6γb heterozygote (K) and pard6γb mutant (L) transgenic fish. (M), double whole mount in situ hybridization (glyt1, dark purple) and immunostaining (GFP, green) of pard6γb mutant. Abbreviation: fp, floor plate.

Expression Data

Genes:	EGFP slc6a9
Antibody:	Ab1-ctnnb
Fish:	sqet33Et sqet33Et/+; sqKR15Et/sqKR15Et sqet33Et; sqKR15Et/+
Anatomical Terms:	central canal roof plate spinal cord region spinal cord glial cell
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sqet33Et/+; sqKR15Et/sqKR15Et
Observed In:	central canal roof plate spinal cord region roof plate spinal cord region cell projection spinal cord development
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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