FIGURE

Fig. 7

ID

ZDB-FIG-110720-76

Publication

Xiao et al., 2011 - Assembly of Lamina-Specific Neuronal Connections by Slit Bound to Type IV Collagen

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Fig. 7

Radial Glia Are Required for the Surface Localization of Slit in the TectumOptical sections of 4 dpf hsp70l:Slit2-GFP tecta. Larvae were heat shocked at 79 hpf and imaged at 96 hpf.(A and B) Radial glia were labeled with Gal4^s1082t and UAS:Kaede. Kaede was photoconverted to red. (A2) and (B2) show higher magnifications of the areas marked with dashed lines in (A) and (B), respectively. Arrows point at radial glial endfeet in the WT (A and A2). Triangles point at the distal ends of radial glia in drg^s510 (B and B2).(C and D) Radial glia were labeled with Gal4s1061t and UAS:nfsb-mCherry. (C) shows control larva treated with 1% DMSO (C2, higher magnification). (D) shows larva treated with 10mM MTZ (D2, higher magnification). Arrows point at radial glial endfeet (C and C2). Arrowheads point at degenerated radial glia (D2).(E) Densitometric plots of Slit2:GFP signal intensity from the areas indicated by yellow rectangles. Measurement and normalization as in Figures 6M and 6N. Scale bars represent 20 μm. See also Figure S5.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 146(1), Xiao, T., Staub, W., Robles, E., Gosse, N.J., Cole, G.J., and Baier, H., Assembly of Lamina-Specific Neuronal Connections by Slit Bound to Type IV Collagen, 164-176, Copyright (2011) with permission from Elsevier. Full text @ Cell