ZFIN Figure: Gregory-Evans et al., 2011, Fig. 5

ZFIN ID: ZDB-FIG-110712-76

ZFIN ID: ZDB-FIG-110712-76

Gregory-Evans et al., 2011 - Gene-specific differential response to anti-apoptotic therapies in zebrafish models of ocular coloboma. Molecular Vision 17:1473-1484

ADDITIONAL FIGURES

PHENOTYPE:

Fish:	lamb1a^m189/m189
Condition:	chemical treatment by environment: curcumin
Observed In:	eye eye apoptotic process lens lens development in camera-type eye
Stage:	Day 6

Fig. 5

Effect of diferuloylmethane on cell death in gup mutant larvae. A: Comparison of TUNEL-positive cells in coronal cryosections of the eye from mutant gup embryos (left), mutant gup embryos treated with 5 μM diferuloylmethane (center) and wildtype embryos (right) at 6 dpf. Histological sections counterstained with methyl green. B: Representative western blot of lysates from untreated mutant larvae (gup), mutant larvae treated with 5 μM diferuloylmethane (gup+D) and wildtype larvae (wt). Upper lanes, correspond to cleaved caspase-3; lower lanes, corresponding gapdh loading controls.

Gene Expression Details No data available

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
lamb1a^m189/m189	control	Day 6	eye apoptotic process increased occurrence, abnormal
lamb1a^m189/m189	chemical treatment by environment: curcumin	Day 6	eye apoptotic process occurrence, ameliorated
		Day 6	eye lacks all parts of type lens, abnormal
		Day 6	lens development in camera-type eye arrested, abnormal

Acknowledgments:
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