ZFIN ID: ZDB-FIG-110712-76
Gregory-Evans et al., 2011 - Gene-specific differential response to anti-apoptotic therapies in zebrafish models of ocular coloboma. Molecular Vision   17:1473-1484
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 6

Fig. 5

Effect of diferuloylmethane on cell death in gup mutant larvae. A: Comparison of TUNEL-positive cells in coronal cryosections of the eye from mutant gup embryos (left), mutant gup embryos treated with 5 μM diferuloylmethane (center) and wildtype embryos (right) at 6 dpf. Histological sections counterstained with methyl green. B: Representative western blot of lysates from untreated mutant larvae (gup), mutant larvae treated with 5 μM diferuloylmethane (gup+D) and wildtype larvae (wt). Upper lanes, correspond to cleaved caspase-3; lower lanes, corresponding gapdh loading controls.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lamb1am189/m189 control Day 6 eye apoptotic process increased occurrence, abnormal
lamb1am189/m189 chemical treatment by environment: curcumin Day 6 eye apoptotic process occurrence, ameliorated
Day 6 eye lacks all parts of type lens, abnormal
Day 6 lens development in camera-type eye arrested, abnormal
Acknowledgments:
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