ZFIN ID: ZDB-FIG-110712-74
Gregory-Evans et al., 2011 - Gene-specific differential response to anti-apoptotic therapies in zebrafish models of ocular coloboma. Molecular Vision   17:1473-1484
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 6

Fig. 3

Representative images of drug treatment in gup mutants at 6 dpf. A, C, E, G, I: Enlarged wholemount of the eye, scale=200 μm, and wholemount larvae, scale=500 μm. B, D, F, H, J: corresponding TUNEL stained wholemount of PTU-treated eye and larvae. A, B: Wildtype zebrafish showing normal morphology and minimal apoptosis in the eye and wholemount. C, D: Untreated gup mutants displaying large coloboma in ventral aspect of the eye, TUNEL-positive labeled tissue at the site of the unfused optic fissure and throughout the whole fish. E, F: gup mutants treated with 5 μM diferuloylmethane (+D) showing small colobomatous defect, minimal TUNEL-positive staining in the eye, and reduced levels in the whole larvae compared to untreated mutants. G, H: gup mutants treated with 300 μM zVAD-fmk (+V) showing small colobomatous defect, minimal TUNEL-positive staining in the eye, and reduced levels in the whole larvae compared to untreated mutants. I, J: gup mutants treated with 300 μM zFA-fmk (+F) showing large colobomatous defect and TUNEL-positive staining at the site of the unfused fissure.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lamb1am189/m189 control Day 6 closure of optic fissure decreased process quality, abnormal
Day 6 eye apoptotic process increased occurrence, abnormal
Day 6 optic fissure increased width, abnormal
Day 6 whole organism apoptotic process increased occurrence, abnormal
lamb1am189/m189 chemical treatment by environment: curcumin Day 6 closure of optic fissure process quality, ameliorated
Day 6 eye apoptotic process occurrence, ameliorated
Day 6 optic fissure width, ameliorated
Day 6 whole organism apoptotic process occurrence, ameliorated
lamb1am189/m189 chemical treatment by environment: Z-Val-Ala-Asp(OMe)-CH2F Day 6 closure of optic fissure process quality, ameliorated
Day 6 eye apoptotic process occurrence, ameliorated
Day 6 optic fissure width, ameliorated
Day 6 whole organism apoptotic process occurrence, ameliorated
Acknowledgments:
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