FIGURE

Fig. 2

ID

ZDB-FIG-101123-25

Publication

Lugo-Villarino et al., 2010 - Identification of dendritic antigen-presenting cells in the zebrafish

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Fig. 2

Enrichment of zebrafish DCs by flow cytometry and lectin-binding affinity. Single-cell suspensions were prepared from WKM and labeled with PNA. (A) Contour plot (Left) demonstrates the distribution of cells in WKM by light scatter: erythrocytes (red), lymphocytes (blue), precursors (purple), myelomonocytes (green), and eosinophils (orange). Histogram (Right) shows PNA binding (black unshaded histogram) and negative control (shaded gray histogram) within the myelomonocyte gate. (B) Cytospin analysis of PNA^hi myelomonocytes purified from WKM and stained with WG: neutrophils (i), monocytes (ii), Mφs (iii), and DCs (iv). (C) DC-like cells isolated from other organs and peripheral sites based on PNA binding: skin (i), gut (ii), IPEX (iii), and gills (iv). (Scale bar: 5 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA