FIGURE

Fig. 3

ID

ZDB-FIG-101123-26

Publication

Lugo-Villarino et al., 2010 - Identification of dendritic antigen-presenting cells in the zebrafish

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Fig. 3

Morphological characterization of zebrafish DCs. PNA^hi myelomonocytes were isolated from WKM (A and B) or skin (C) by FACS. (A) Cells were stained for WG, H&E, AP (magenta precipitate), NAE (black precipitate), MPX (brown precipitate), PAS (red precipitate), and TB (purple precipitate). (Upper) Putative DCs for each stain. (Lower) Positive staining controls: Mφs (i-iii), monocyte (iv), neutrophil (v), eosinophil (vi), and mast cell (vii). (Scale bar: 5 μm.) (B) TEM was performed to examine the ultrastructure of PNA^hi myelomonocytes. (Upper) DCs. (Lower) Other leukocytes: Mφ (i), neutrophil (ii), and eosinophil (iii). Denoted features include nucleus (N), apoptotic corpse (AC), cigar-shaped granules (red arrowheads), round, electron-dense granules (blue arrowheads), and mitochondria (green arrowheads). (Scale bar: 1 μm.) (C) TEM analysis of Birbeck-like granules from PNA^hi DCs in skin. Magnified granule regions: vacuole (red arrowheads) and rod structure (blue arrowheads). (Scale bar: 200 nm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA