FIGURE

Fig. 4

ID

ZDB-FIG-101123-27

Publication

Lugo-Villarino et al., 2010 - Identification of dendritic antigen-presenting cells in the zebrafish

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Fig. 4

Further enrichment of zebrafish DCs for functional analyses. (A) Single-cell suspensions were prepared from mpx:eGFP transgenic WKM and labeled with PNA. Myelomonocytes (green gate) were divided into mpx^- (black box) and mpx⁺ (blue box). These populations were further subdivided into mpx^- PNA^- (gray box), mpx^- PNA⁺ (red box), and mpx⁺ PNA⁺ (blue box) fractions. (B) After 4 h of culture, mpx^- PNA^- (gray), mpx^- PNA⁺ (red), and mpx⁺ PNA⁺ (blue) fractions were collected and stained with MGG to assess cell morphology. Differentials are presented as mean ± SD, n = 6. (C) mpx^- PNA⁺ (red bar), mpx⁺ PNA⁺ (blue bar), and mpx^- PNA^- (black bar) myelomonocyte fractions were cultured for 16 h with and without LPS. The abundance of il-12p40, csf1r, and iclp1 transcripts were measured by qPCR. Expression is presented as fold induction over nonstimulated controls. Asterisks denote populations with no detectable expression of the transcript. Bars represent mean, n = 3. Error bars represent SD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA