ZFIN ID: ZDB-FIG-100525-4
Sarmah et al., 2010 - Sec24D-dependent transport of extracellular matrix proteins is required for zebrafish skeletal morphogenesis. PLoS One   5(4):e10367 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 3 Sec24D-deficient cartilage contains sparse ECM and chondrocytes with distended ER.

(A–H) Electron micrographs show mature wild-type chondrocytes (cells 1–3 in A) are separated by ECM and contain dense ER membranes (A, E, arrows). In bulm494 mutants (B,F,H), cartilage matrix is sparse and large amounts of electron-dense material accumulate in the rough ER (asterisks, cells marked 1–6 in B). Wild-type chondrocytes (C) are devoid of cell-cell contacts, whereas the majority of bulm494 mutant cells (D) contain adherens junctions (arrowheads). bulm494 chondrocytes (F) contain both normal ER cisternae (arrows) and swollen, ribosome studded, ER (asterisks). sec24d-deficient cells (H) contain smaller and disorganized Golgi complexes (blue arrows) compared to wild-types (G). (I,J) Single-pass confocal images of C6-NBD ceramide stained chondrocytes of wild-type (I) and sec24d/bulm606 mutant (J) embryos at 4 dpf. (K,L) Adherens junctions are marked by β-catenin (red) in bulldog Meckel′s cartilage (arrowheads), but are mostly absent in wild types (K). (M,N) Phalloidin (red) shows regular cortical distribution of polymerized actin in wild types (M), but uneven cellular distribution in bulm606 cartilage (arrowheads) (N). Nuclei are stained with TOPRO-3 (blue). Abbreviations: m, mitochondria; n, nucleus; ECM, extracellular matrix. Scale bars: 2 μM (A,B,E); 500 nm (F–H); 5 μM (I–N).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sec24dm494/m494 (AB) standard conditions Day 4 chondrocyte differentiation arrested, abnormal
Day 4 chondrocyte endoplasmic reticulum lumen swollen, abnormal
Day 4 chondrocyte Golgi apparatus decreased size, abnormal
Day 4 chondrocyte Golgi apparatus disorganized, abnormal
Day 4 chondrocyte undifferentiated, abnormal
Day 4 pharyngeal arch cartilage extracellular matrix decreased volume, abnormal
sec24dm606/m606 (AB) standard conditions Day 4 chondrocyte actin filament disorganized, abnormal
Day 4 chondrocyte adherens junction increased amount, abnormal
Day 4 chondrocyte differentiation arrested, abnormal
Day 4 Meckel's cartilage adherens junction increased amount, abnormal
Day 4 pharyngeal arch cartilage chondrocyte disorganized, abnormal
Day 4 pharyngeal arch cartilage chondrocyte malformed, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One