ZFIN ID: ZDB-FIG-100525-5
Sarmah et al., 2010 - Sec24D-dependent transport of extracellular matrix proteins is required for zebrafish skeletal morphogenesis. PLoS One   5(4):e10367 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 4 Analysis of chondrocyte shape and numbers in bulldog mutants.

(A) The number of cells in the ceratohyals at 5 dpf is not significantly different between wild-type and bulldog embryos (counted in single optical plane of Alcian blue stained preparations, six different animals each). (B) In contrast, the number of cells spanning the entire width of the ceratohyal at 5 dpf is notably higher in bulldog. (C,D) Single-pass confocal images of the Meckel′s cartilage in live embryos marked with membrane tethered GFP tracer. Bulldog mutants (D) show multiple stacked chondrocytes as compared to a single spanning cell in wild-types (C). (E) The average chondrocyte width is comparable between wild-type and bulldog, whereas the length and the length-to-width ratio are significantly lower in the mutants. Cellular dimensions were counted in Meckel′s (m) and ceratohyal (ch) cartilages in three different live embryos at 3 dpf. The number of cells used for measurements is indicated in the right graph (E). * denotes p<0.0001; **, p<0.0001; $$, p<0.003; ##, p<0.0001.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sec24dm494/m494 (AB) standard conditions Protruding-mouth ceratohyal cartilage chondrocyte obtuse, abnormal
Protruding-mouth Meckel's cartilage chondrocyte disorganized, abnormal
Protruding-mouth Meckel's cartilage chondrocyte length, abnormal
Protruding-mouth Meckel's cartilage chondrocyte obtuse, abnormal
Day 5 ceratohyal cartilage chondrocyte normal amount, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One