ZFIN ID: ZDB-FIG-100525-3
Sarmah et al., 2010 - Sec24D-dependent transport of extracellular matrix proteins is required for zebrafish skeletal morphogenesis. PLoS One   5(4):e10367 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 8-cell to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 2 sec24d mRNA expression during embryogenesis and molecular analysis of the bulldog mutation.

(A) RT-PCR assay shows maternally deposited sec24d transcripts at 1 and 2 hpf and increasing levels thereafter. β-actin served as control. (B–H) Expression patterns of sec24d during zebrafish development using whole mount in situ hybridization staining of wild-type embryos. The cross section through the palatoquadrate (pq, arrowheads) at 3 dpf (I) shows robust riboprobe staining (h: hours, d: days). (J–O) Expression patterns of col2α1 (J,K) and sox9a (L,M) at 3 dpf. The expression is slightly upregulated in the posterior pharyngeal cartilages of bulm606 embryos. The Collagen-specific chaperone hsp47 (N,O) transcript is elevated in craniofacial skeleton (arrowheads), fins and the notochord. Arrowheads point to the jaw region in panels (G) through (O). (P) Relative sec24d sec24c, bip and sil1 mRNA levels were assessed by quantitative real-time PCR and adjusted against β-actin using total RNA samples from 4 dpf embryos. In bulm494, mRNA levels for bip and sil1 are induced 5-fold, whereas sec24d mRNA levels are reduced 0.7-fold. *, p<0.02; **, p<0.005. Abbreviation: b, brain, o, optic nerve, ot, otic capsule, r, retina. Anterior (D–O) is to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col2a1a AB standard conditions Protruding-mouth otic vesicle ISH
Protruding-mouth pharyngeal arch cartilage chondrocyte ISH
sec24dm606/m606 (AB) standard conditions Protruding-mouth otic vesicle ISH
Protruding-mouth pharyngeal arch cartilage chondrocyte ISH
sec24d AB standard conditions 8-cell whole organism ISH
4-cell to Day 5 whole organism RTPCR
Shield whole organism ISH
5-9 somites central nervous system ISH
5-9 somites immature eye ISH
20-25 somites brain ISH
20-25 somites immature eye ISH
20-25 somites notochord ISH
20-25 somites somite ISH
Long-pec brain ISH
Long-pec eye ISH
Long-pec somite ISH
Protruding-mouth brain ISH
Protruding-mouth eye ISH
Protruding-mouth myotome ISH
Protruding-mouth palatoquadrate cartilage ISH
Protruding-mouth pharyngeal arch cartilage ISH
Day 4 brain ISH
Day 4 eye ISH
Day 4 myotome ISH
Day 4 pharyngeal arch cartilage ISH
serpinh1b AB standard conditions Protruding-mouth otic vesicle ISH
sec24dm606/m606 (AB) standard conditions Protruding-mouth notochord ISH
Protruding-mouth otic vesicle ISH
Protruding-mouth pectoral fin ISH
Protruding-mouth pharyngeal arch cartilage ISH
sox9a AB standard conditions Protruding-mouth pharyngeal arch cartilage chondrocyte ISH
sec24dm606/m606 (AB) standard conditions Protruding-mouth pharyngeal arch cartilage chondrocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sec24dm606/m606 (AB) standard conditions Protruding-mouth collagen biosynthetic process increased rate, abnormal
Protruding-mouth pharyngeal arch cartilage physical object quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One