FIGURE

Fig. 1

ID

ZDB-FIG-091221-1

Publication

Schwend et al., 2009 - Zebrafish con/disp1 reveals multiple spatiotemporal requirements for Hedgehog-signaling in craniofacial development

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Fig. 1

con/disp1 mutants display defective cartilage development in the PA which is partly rescued by RNA encoding non lipid-modified Shh. (A,B) Lateral views of live larvae show a reduction in head tissue and a deficit in jaw outgrowth (arrowhead) in con/disp1 mutant larvae at 5 dpf. (C) Ventral view of 96 hpf Alcian blue-stained wild type cartilages. (D) con/disp1 mutant cartilages reveal reduced, hypoplastic mandibular and hyoid arch cartilage elements and a complete absence of the cb 1-5 elements and midline-forming bh cartilage. (E,F) Lateral view of jaw cartilages reveals malformed joints (arrows denote joint site), fewer chondrocytes contributing to the pq cartilage element and a complete loss of the ptp and sy cartilage element in con/disp1 mutant (arrowhead denotes presence of cartilage, asterisk denotes cartilage absence). (G) Injection of 150 pg shhN mRNA into genotype-confirmed con/disp1 mutant mostly restores cartilage elements to wild type state (arrow indicates unilateral rescue in cb 4-5 elements), while (H) con/disp1 mutant injected with 150 pg shhFL mRNA are phenotypically similar to noninjected con/disp1 larvae. Scale bar: 50 μM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	disp1^tm15a/tm15a
Observed In:	basihyal cartilage face morphogenesis hyosymplectic cartilage mandibular arch skeleton palatoquadrate cartilage pharyngeal arch 2 skeleton pharyngeal arch 3-7 skeleton
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.