ZFIN ID: ZDB-FIG-091214-13
Bonkowsky et al., 2008 - Domain-specific regulation of foxP2 CNS expression by lef1. BMC Developmental Biology   8:103 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-25

Fig. 6 lef1 is necessary for expression from foxP2-enhancerA.1, foxP2-enhancerB, and foxP2-enhancerD. Whole-mount gfp in situs at 36 hpf; anterior to left, dorsal up, eyes removed. Scale bar = 50 μm. Conditions (wild type, wt; or lef1 morphant, lef1 MO) are shown above the panels, enhancer names to left. (Abbreviations: dd, dorsal diencephalon; hb, hindbrain; tec, tectum; tel, telencephalon.) (A) Tg(foxP2-enhancerA.1:EGFP)zc44 expresses in telencephalon, tectum, and hindbrain. (B) Tg(foxP2-enhancerA.1:EGFP)zc44 embryo injected with lef1 morpholino lacks GFP expression in tectum and hindbrain (arrows), although telencephalic expression persists. (C) Tg(foxP2-enhancerB:EGFP)zc41 expresses in telencephalon and hindbrain. (D) Tg(foxP2-enhancerB:EGFP)zc41 embryo injected with lef1 morpholino lacks GFP expression in the hindbrain (arrow), but telencephalic expression is present. (E) Tg(foxP2-enhancerD:EGFP)zc47 expresses in dorsal diencephalon and tectum. (F) Tg(foxP2-enhancerD:EGFP)zc47 embryo injected with lef1 morpholino lacks GFP expression in the tectum (arrow), but dorsal diencephalic expression persists. (G) Tg(elavl3:EGFP)zf8 embryo shows GFP expression in all post-mitotic neurons. (H) Tg(elavl3:EGFP)zf8 embryo injected with lef1 morpholino still has GFP expression in the tectum and hindbrain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP knu3Tg control Prim-25 hindbrain ISH
Prim-25 midbrain hindbrain boundary ISH
Prim-25 neuron ISH
Prim-25 optic tectum ISH
knu3Tg + MO2-lef1 standard conditions Prim-25 hindbrain ISH
Prim-25 midbrain hindbrain boundary ISH
Prim-25 neuron ISH
Prim-25 optic tectum ISH
zc41Tg control Prim-25 hindbrain ISH
Prim-25 telencephalon ISH
zc41Tg + MO2-lef1 standard conditions Prim-25 Not Detected hindbrain ISH
Prim-25 telencephalon ISH
zc44Tg control Prim-25 hindbrain ISH
Prim-25 optic tectum ISH
Prim-25 telencephalon ISH
zc44Tg + MO2-lef1 standard conditions Prim-25 Not Detected hindbrain ISH
Prim-25 Not Detected optic tectum ISH
Prim-25 telencephalon ISH
zc47Tg control Prim-25 diencephalon ISH
Prim-25 optic tectum ISH
zc47Tg + MO2-lef1 standard conditions Prim-25 diencephalon ISH
Prim-25 Not Detected optic tectum ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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