ZFIN ID: ZDB-FIG-091214-16
Bonkowsky et al., 2008 - Domain-specific regulation of foxP2 CNS expression by lef1. BMC Developmental Biology   8:103 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec

Fig. S2 lef1 is necessary for expression from foxP2-enhancerA.1, foxP2-enhancerB, and foxP2-enhancerD. Whole-mount confocal images at 52 hpf (except foxP2-enhancerD), lateral view, anterior left, dorsal up. Conditions (wild type- wt, or lef1 morphant- lef1 MO) are shown above the panels, while the enhancer name is to the left of the panels. (Abbreviations: hb: hindbrain; tec: tectum; tel: telencephalon.) (A) Tg(foxP2-enhancerA.1:EGFP)zc44 expresses in telencephalon, tectum, and hindbrain. (B) Tg(foxP2-enhancerA.1:EGFP)zc44 embryo injected with lef1 morpholino: GFP expression is absent in the tectum and hindbrain (arrows), although telencephalic expression persists. (C) Tg(foxP2-enhancerB:EGFP)zc41 expresses in telencephalon, tectum, and hindbrain. (D) Tg(foxP2-enhancerB:EGFP)zc41 embryo injected with lef1 morpholino: GFP expression is absent in the hindbrain (arrow), but still present in the telencephalon. (E) Tg(foxP2-enhancerD:EGFP)zc47 expresses in tectum. (F) Tg(foxP2-enhancerD:EGFP)zc47 embryo injected with lef1 morpholino: GFP expression is absent in the tectum (arrow). (G) Tg(elavl3:EGFP)zf8 embryo shows GFP expression in all post-mitotic neurons. (H) Tg(elavl3:EGFP)zf8 embryo injected with lef1 morpholino: GFP expression is still present in the tectum and hindbrain. (I) Tg(pax2a:GFP)e1 embryo shows expression in the MHB and hindbrain. (J) Tg(pax2a:GFP)e1 embryo injected with lef1 morpholino has persistent expression in the MHB and hindbrain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP knu3Tg control Long-pec hindbrain IFL
Long-pec neuron IFL
Long-pec optic tectum IFL
knu3Tg + MO2-lef1 standard conditions Long-pec hindbrain IFL
Long-pec neuron IFL
Long-pec optic tectum IFL
zc41Tg control Long-pec hindbrain IFL
Long-pec telencephalon IFL
zc41Tg + MO2-lef1 standard conditions Long-pec Not Detected hindbrain IFL
Long-pec telencephalon IFL
zc44Tg control Long-pec hindbrain IFL
Long-pec optic tectum IFL
Long-pec telencephalon IFL
zc44Tg + MO2-lef1 standard conditions Long-pec Not Detected hindbrain IFL
Long-pec Not Detected optic tectum IFL
Long-pec telencephalon IFL
zc47Tg control Long-pec optic tectum IFL
zc47Tg + MO2-lef1 standard conditions Long-pec Not Detected optic tectum IFL
GFP e1Tg control Long-pec hindbrain IFL
Long-pec midbrain hindbrain boundary IFL
e1Tg + MO2-lef1 standard conditions Long-pec hindbrain IFL
Long-pec midbrain hindbrain boundary IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.