ZFIN ID: ZDB-FIG-090717-7
Lin et al., 2009 - The transcription factor six1a plays an essential role in the craniofacial myogenesis of zebrafish. Developmental Biology   331(2):152-166 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 7 Similar to Six1a, Pax3 is required for the development of mr, ir and all arch muscles. Embryos derived from the wild-type strain (A–F) and from the transgenic line Tg(α-actin:RFP) (G–R) were examined. Whole-mount in situ hybridization was used to detect the expressions of myf5 and myod at 48 hpf in the wild-type embryos, which served as a control group (A, D). Compared to the control group, embryos injected with 3 ng of pax3-MO exhibited a normal expression of myf5 in head muscle primordia at 48 hpf (B), but these embryos expressed myod only in so/sr, lr, io, and some remnant branchial muscles (E). Embryos injected with 10 ng of eya1-MO expressed myf5 and myod normally in head muscle primordia at 48 hpf (C, F). We also noticed that the RFP expression of embryos injected with six1a-MO was similar to that of control group at 72 hpf (G vs. J). The RFP signal appeared in the so, io, sr, lr and some remnant branchial muscles in pax3-MO-injected embryos, which was similar to that of the six1a morphants at 72 hpf (G vs. H and J vs. K). The RFP signal appeared in all cranial muscles of the eya1-MO-injected embryos at 72 hpf (I and L). By co-injection of 10 ng of pax3-MO with 150 pg of six1a mRNA (M, P), 100 pg of myf5 mRNA (N, Q) or 50 pg of myod mRNA (O, R), we found that the defective expressions of six1a, myf5 and myod could not be rescued in the pax3 morphants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myf5 WT control Long-pec dorsal oblique extraocular muscle ISH
Long-pec pharyngeal arch 2 ISH
Long-pec pharyngeal arch 3 ISH
Long-pec ventral oblique extraocular muscle ISH
WT + MO2-eya1 standard conditions Long-pec dorsal oblique extraocular muscle ISH
Long-pec pharyngeal arch 2 ISH
Long-pec pharyngeal arch 3 ISH
Long-pec ventral oblique extraocular muscle ISH
WT + MO2-pax3a standard conditions Long-pec dorsal oblique extraocular muscle ISH
Long-pec pharyngeal arch 2 ISH
Long-pec pharyngeal arch 3 ISH
Long-pec ventral oblique extraocular muscle ISH
myod1 WT control Long-pec adductor hyohyoid ISH
Long-pec adductor mandibulae ISH
Long-pec constrictor dorsalis ISH
Long-pec dorsal oblique extraocular muscle ISH
Long-pec dorsal rectus ISH
Long-pec hyohyoideus ISH
Long-pec lateral rectus ISH
Long-pec mandibular muscle ISH
Long-pec medial rectus ISH
Long-pec sternohyoid ISH
Long-pec ventral oblique extraocular muscle ISH
Long-pec ventral rectus ISH
WT + MO2-eya1 standard conditions Long-pec adductor hyohyoid ISH
Long-pec adductor mandibulae ISH
Long-pec constrictor dorsalis ISH
Long-pec dorsal oblique extraocular muscle ISH
Long-pec dorsal rectus ISH
Long-pec hyohyoideus ISH
Long-pec lateral rectus ISH
Long-pec mandibular muscle ISH
Long-pec medial rectus ISH
Long-pec sternohyoid ISH
Long-pec ventral oblique extraocular muscle ISH
Long-pec ventral rectus ISH
WT + MO2-pax3a standard conditions Long-pec Not Detected adductor hyohyoid ISH
Long-pec Not Detected adductor mandibulae ISH
Long-pec Not Detected constrictor dorsalis ISH
Long-pec dorsal oblique extraocular muscle ISH
Long-pec dorsal rectus ISH
Long-pec Not Detected hyohyoideus ISH
Long-pec lateral rectus ISH
Long-pec Not Detected mandibular muscle ISH
Long-pec Not Detected medial rectus ISH
Long-pec Not Detected sternohyoid ISH
Long-pec ventral oblique extraocular muscle ISH
Long-pec Not Detected ventral rectus ISH
RFP twu3Tg + MO1-six1b standard conditions Protruding-mouth dorsal oblique extraocular muscle IFL
Protruding-mouth dorsal rectus IFL
Protruding-mouth lateral rectus IFL
Protruding-mouth ventral oblique extraocular muscle IFL
twu3Tg + MO2-eya1 standard conditions Protruding-mouth head muscle ISH
twu3Tg + MO2-pax3a standard conditions Protruding-mouth dorsal oblique extraocular muscle ISH
Protruding-mouth dorsal rectus ISH
Protruding-mouth lateral rectus ISH
Protruding-mouth ventral oblique extraocular muscle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-pax3a standard conditions Long-pec dorsal oblique extraocular muscle present, normal
Long-pec dorsal rectus present, normal
Long-pec lateral rectus present, normal
Long-pec medial rectus aplastic, abnormal
Long-pec pharyngeal arch muscle aplastic, abnormal
Long-pec sternohyoid aplastic, abnormal
Long-pec ventral oblique extraocular muscle present, normal
Long-pec ventral rectus aplastic, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 331(2), Lin, C.Y., Chen, W.T., Lee, H.C., Yang, P.H., Yang, H.J., and Tsai, H.J., The transcription factor six1a plays an essential role in the craniofacial myogenesis of zebrafish, 152-166, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.