ZFIN ID: ZDB-FIG-090717-6
Lin et al., 2009 - The transcription factor six1a plays an essential role in the craniofacial myogenesis of zebrafish. Developmental Biology   331(2):152-166 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-25 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 6 The function of Six1a in branchial muscle development requires Tbx1 and Myf5 to play a specification role on arch muscle cell fate. Embryos derived from the wild-type strain (A–D) and from the transgenic line Tg(α-actin:RFP) (E–H) were examined at lateral view. Whole-mount in situ hybridization was used to detect the tbx1 expression in arch muscle and cranial mesoderm in wild-type embryos at 36 hpf (A). Compared to the above control embryos, the expressions of six1a (B), myf5 (C) and myod (D) in the 10 ng group of tbx1-MO-injected embryos were lost in arch muscles, but retained in extraocular muscles. We also observed that all the pharyngeal arch muscles were lost, but that 6 extraocular muscles developed normally in the 10 ng group of tbx1-MO-injected embryos derived from Tg(α-actin:RFP) at 72 hpf (E). With co-injection of 10 ng of tbx1-MO and either 150 pg of six1a mRNA (F), 100 pg of myf5 mRNA (G) or 50 pg of myod mRNA (H) in embryos, we found that only myf5 mRNA enabled embryos to rescue the RFP expression in lap, do, ah, ao, am, ima/imp and ih/hh (marked in green typeface of G). The heart defect induced by injection of tbx1-MO is labeled with a white star. ah, adductor hyoideus; am, adductor mandibulae; ao, adductor operculi; do, dilator operculi; hh, hyohyoideus; ih, interhyoideus; ima, intermandibularis anterior; imp, intermandibularis posterior; io, inferior oblique; ir, inferior rectus; lap, levator arcus palatini; lr, lateral rectus; mr, medial rectus; sh, sternohyoideus; so, superior oblique and sr, superior rectus. Embryos were all lateral views.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myf5 WT + MO1-tbx1 standard conditions Prim-25 dorsal oblique extraocular muscle ISH
Prim-25 Not Detected pectoral fin musculature ISH
Prim-25 ventral oblique extraocular muscle ISH
myod1 WT + MO1-tbx1 standard conditions Prim-25 dorsal rectus ISH
Prim-25 lateral rectus ISH
Prim-25 medial rectus ISH
Prim-25 Not Detected pectoral fin musculature ISH
Prim-25 ventral rectus ISH
RFP twu3Tg + MO1-tbx1 standard conditions Protruding-mouth dorsal oblique extraocular muscle ISH
Protruding-mouth dorsal rectus ISH
Protruding-mouth lateral rectus ISH
Protruding-mouth medial rectus ISH
Protruding-mouth sternohyoid ISH
Protruding-mouth ventral oblique extraocular muscle ISH
Protruding-mouth ventral rectus ISH
six1b WT + MO1-tbx1 standard conditions Prim-25 dorsal rectus ISH
Prim-25 medial rectus ISH
Prim-25 Not Detected pharyngeal musculature ISH
tbx1 WT standard conditions Prim-25 pharyngeal musculature ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
twu3Tg + MO1-tbx1 standard conditions Protruding-mouth pharyngeal arch muscle aplastic, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 331(2), Lin, C.Y., Chen, W.T., Lee, H.C., Yang, P.H., Yang, H.J., and Tsai, H.J., The transcription factor six1a plays an essential role in the craniofacial myogenesis of zebrafish, 152-166, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.