ZFIN ID: ZDB-FIG-090717-5
Lin et al., 2009 - The transcription factor six1a plays an essential role in the craniofacial myogenesis of zebrafish. Developmental Biology   331(2):152-166 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-25 to Protruding-mouth

Fig. 5 Injection of six1a-, myf5-and myod mRNA enables embryos to rescue trunk migratory head muscle sternohyoideus (sh) defect in six1a morphants. Dorsal views of embryos derived either from the transgenic line Tg(α-actin:RFP) (A, D, G, J, M) or from the wild-type (B, C, E, F, H, I, K, L). The RFP expression in the embryos derived from the transgenic line at 72 hpf (A) and the detection of myf5 and myod by whole-mount in situ hybridization at 36 hpf (B, C) served as control groups. Injection of embryos with either 8 ng of six1a-MO alone (D–F) or co-injection with 8 ng of six1a-MO and 150 pg of six1a mRNA (G–I), 100 pg of myf5 mRNA (J–L) or 50 pg of myod mRNA (M–O) were examined. RFP, myf5 and myod were not detected in sh, fb, or phm primordia in the six1a morphants (D–F); however, co-injection of six1a mRNA enabled embryos to rescue the defective expressions of RFP, myf5 and myod in sh, fb and phm primordia induced by six1a-MO at 48 hpf (H, I) and at 72 hpf (G). Meanwhile, injection of myf5 mRNA enabled embryos to rescue the defective expressions of RFP, myod and myogenin in sh, fb and phm primordia induced by six1a-MO at 48 hpf (K, L) and at 72 hpf (J). Injection of myod mRNA enabled embryos to rescue the defective expressions of RFP, myf5 and myogenin in sh, fb and phm primordia induced by six1a-MO at 48 hpf (N, O) and at 72 hpf (M). fb, fin bud; phm, posterior hypoaxial muscle; and sh, sternohyoideus.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myf5 WT control Prim-25 hypaxialis ISH
Prim-25 pectoral fin musculature ISH
WT + MO1-six1b standard conditions Prim-25 Not Detected hypaxialis ISH
Prim-25 Not Detected pectoral fin musculature ISH
myod1 WT control Prim-25 hypaxialis ISH
Prim-25 pectoral fin musculature ISH
WT + MO1-six1b standard conditions Prim-25 Not Detected hypaxialis ISH
Prim-25 Not Detected pectoral fin musculature ISH
RFP twu3Tg control Protruding-mouth hypaxialis IFL
Protruding-mouth pectoral fin musculature IFL
Protruding-mouth sternohyoid IFL
twu3Tg + MO1-six1b standard conditions Protruding-mouth Not Detected hypaxialis IFL
Protruding-mouth Not Detected pectoral fin musculature IFL
Protruding-mouth Not Detected sternohyoid IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 331(2), Lin, C.Y., Chen, W.T., Lee, H.C., Yang, P.H., Yang, H.J., and Tsai, H.J., The transcription factor six1a plays an essential role in the craniofacial myogenesis of zebrafish, 152-166, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.