Fig. 2 Expression profiles of zebrafish synuclein-related transcripts in various adult tissues and at different developmental stages by reverse transcriptase-polymerase chain reaction (RT-PCR). A,B: RT-PCR was performed with gene-specific primers. β-actin bands were used to normalize the amount of cDNA prepared from different tissues (A) and from different developmental stages (B). The developmental expression profile of each gene was examined in embryos from 4 hours postfertilization (hpf) to 5 dpf.