ZFIN Figure: Chen et al., 2009, Fig. 4

ZFIN ID: ZDB-FIG-090310-17

ZFIN ID: ZDB-FIG-090310-17

Chen et al., 2009 - Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene. Developmental dynamics : an official publication of the American Association of Anatomists 238(3):746-754 Full text @ Dev. Dyn.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	cadps2 GFP kctd12.1 kctd12.2 sncgb spon1b
Fish:	as4Tg
Anatomical Terms:	habenula hindbrain lens midbrain posterior lateral line ganglion retinal ganglion cell layer
Stage:	Protruding-mouth

Fig. 4 Double fluorescence in situ hybridizations of habenula-expressing markers, gfp and sncga in Tg(sncga:GFP) fish. A: Expression domains of sncga (a′), lov (b′), ron (c′), cpd2 (d′) and f-spondin2 (e′) along with gfp (a-e) in habenula in Tg(sncga:GFP) fish at 3 days postfertilization (dpf). a″-e″, merged image of a and a′, b and b′, c and c′; d and d′, and e and e′, respectively. B: Double fluorescence in situ hybridization of sncga and gfp in an Tg(sncga:GFP) fish at 3 dpf. Panel a shows the staining of gfp mRNA, whereas panel b shows the staining of sncga mRNA. Panel c shows the merged image, in which gfp and sncga staining completely overlapped. gcl, retinal ganglion cell layer; GFP, green fluorescent protein; gP, posterior lateral line ganglion; Ha, habenula; hb, hindbrain; l, lens; mb, midbrain.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
cadps2	as4Tg	standard conditions	Protruding-mouth	habenula	ISH
GFP	as4Tg	standard conditions	Protruding-mouth	habenula	ISH
			Protruding-mouth	hindbrain	ISH
			Protruding-mouth	lens	ISH
			Protruding-mouth	midbrain	ISH
			Protruding-mouth	posterior lateral line ganglion	ISH
			Protruding-mouth	retinal ganglion cell layer	ISH
kctd12.1	as4Tg	standard conditions	Protruding-mouth	habenula	ISH
kctd12.2	as4Tg	standard conditions	Protruding-mouth	habenula	ISH
sncgb	as4Tg	standard conditions	Protruding-mouth	habenula	ISH
			Protruding-mouth	hindbrain	ISH
			Protruding-mouth	lens	ISH
			Protruding-mouth	midbrain	ISH
			Protruding-mouth	posterior lateral line ganglion	ISH
			Protruding-mouth	retinal ganglion cell layer	ISH
spon1b	as4Tg	standard conditions	Protruding-mouth	habenula	ISH

Antibody Labeling Details No data available

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.