ZFIN ID: ZDB-FIG-090310-16
Chen et al., 2009 - Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene. Developmental dynamics : an official publication of the American Association of Anatomists   238(3):746-754 Full text @ Dev. Dyn.
Anatomical Terms:
Stage: Protruding-mouth

Fig. 3 The expression pattern of green fluorescent protein (GFP) in transgenic zebrafish Tg(sncga:GFP) line. Microinjection of the expression construct into zebrafish embryos at the one- to two-cell stage and generation of transgenic GFP line by means of Tol2-mediated transgenesis were described in the text. A: Images from Tg(sncga:GFP) transgenic line at 3 days postfertilization (dpf). Images of bright field and fluorescence are merged and shown in panel b, while fluorescence images are shown in panel a. sc, spinal cord; nc, notochord. B: Confocal image analysis. Zebrafish larva of transgenic Tg(sncga:GFP) line at 3 dpf were collected and fixed with 4% paraformaldehyde. After treatment of FocusClear, embryos were subjected to high-resolution confocal image analysis. All embryos are shown with the anterior to the left. Embryos in a, c, d, e are shown in lateral view, while embryos in b are shown in dorsal view. White arrowheads indicated habenulo-interpeduncular projection (panel b) and posterior lateral line projection (panels d, e). C: Whole-mount immunostaining of Tg(sncga:GFP) embryos by polyclonal antibody against GFP. Embryos in a and b are shown in lateral view, while embryos in c are shown in dorsal view. The habenulo-interpeduncular projection was labeled by black arrowheads. D: Expression patterns of sncga mRNA in zebrafish embryo at 3 dpf. gcl, retinal ganglion cell layer; gP, posterior lateral line ganglion; gV, trigeminal ganglion; gVIII, statoacoustic ganglion; gX, vagal ganglion; Ha, habenula; hb, hindbrain; mb, midbrain; IN, interneuron; inl, inner nuclear layer; IPN, interpeduncular nucleus; l, lens; LHa, left-side habenula; MHB, midbrain-hindbrain boundary; RB, Rohon-Beard neuron; RHa, right-side habenula.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP as4Tg standard conditions Protruding-mouth epibranchial ganglion IFL
Protruding-mouth eye IFL
Protruding-mouth habenula IHC
Protruding-mouth habenula IFL
Protruding-mouth hindbrain IFL
Protruding-mouth interpeduncular nucleus medulla oblongata IFL
Protruding-mouth interpeduncular nucleus medulla oblongata IHC
Protruding-mouth midbrain IFL
Protruding-mouth midbrain hindbrain boundary IHC
Protruding-mouth midbrain hindbrain boundary IFL
Protruding-mouth posterior lateral line ganglion IFL
Protruding-mouth spinal cord IFL
Protruding-mouth trigeminal ganglion IFL
sncgb WT standard conditions Protruding-mouth epibranchial ganglion IHC
Protruding-mouth habenula IHC
Protruding-mouth hindbrain IHC
Protruding-mouth midbrain IHC
Protruding-mouth posterior lateral line ganglion IHC
Protruding-mouth retinal ganglion cell layer IHC
Protruding-mouth retinal inner nuclear layer IHC
Protruding-mouth statoacoustic (VIII) ganglion IHC
Antibody Labeling Details No data available
Phenotype Details No data available
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