ZFIN ID: ZDB-FIG-090310-16
Chen et al., 2009 - Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene. Developmental dynamics : an official publication of the American Association of Anatomists   238(3):746-754 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 3 The expression pattern of green fluorescent protein (GFP) in transgenic zebrafish Tg(sncga:GFP) line. Microinjection of the expression construct into zebrafish embryos at the one- to two-cell stage and generation of transgenic GFP line by means of Tol2-mediated transgenesis were described in the text. A: Images from Tg(sncga:GFP) transgenic line at 3 days postfertilization (dpf). Images of bright field and fluorescence are merged and shown in panel b, while fluorescence images are shown in panel a. sc, spinal cord; nc, notochord. B: Confocal image analysis. Zebrafish larva of transgenic Tg(sncga:GFP) line at 3 dpf were collected and fixed with 4% paraformaldehyde. After treatment of FocusClear, embryos were subjected to high-resolution confocal image analysis. All embryos are shown with the anterior to the left. Embryos in a, c, d, e are shown in lateral view, while embryos in b are shown in dorsal view. White arrowheads indicated habenulo-interpeduncular projection (panel b) and posterior lateral line projection (panels d, e). C: Whole-mount immunostaining of Tg(sncga:GFP) embryos by polyclonal antibody against GFP. Embryos in a and b are shown in lateral view, while embryos in c are shown in dorsal view. The habenulo-interpeduncular projection was labeled by black arrowheads. D: Expression patterns of sncga mRNA in zebrafish embryo at 3 dpf. gcl, retinal ganglion cell layer; gP, posterior lateral line ganglion; gV, trigeminal ganglion; gVIII, statoacoustic ganglion; gX, vagal ganglion; Ha, habenula; hb, hindbrain; mb, midbrain; IN, interneuron; inl, inner nuclear layer; IPN, interpeduncular nucleus; l, lens; LHa, left-side habenula; MHB, midbrain-hindbrain boundary; RB, Rohon-Beard neuron; RHa, right-side habenula.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP as4Tg standard conditions Protruding-mouth epibranchial ganglion IFL
Protruding-mouth eye IFL
Protruding-mouth habenula IHC
Protruding-mouth habenula IFL
Protruding-mouth hindbrain IFL
Protruding-mouth interpeduncular nucleus medulla oblongata IFL
Protruding-mouth interpeduncular nucleus medulla oblongata IHC
Protruding-mouth midbrain IFL
Protruding-mouth midbrain hindbrain boundary IHC
Protruding-mouth midbrain hindbrain boundary IFL
Protruding-mouth posterior lateral line ganglion IFL
Protruding-mouth spinal cord IFL
Protruding-mouth trigeminal ganglion IFL
sncgb WT standard conditions Protruding-mouth epibranchial ganglion IHC
Protruding-mouth habenula IHC
Protruding-mouth hindbrain IHC
Protruding-mouth midbrain IHC
Protruding-mouth posterior lateral line ganglion IHC
Protruding-mouth retinal ganglion cell layer IHC
Protruding-mouth retinal inner nuclear layer IHC
Protruding-mouth statoacoustic (VIII) ganglion IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.