FIGURE

Fig. 4

ID

ZDB-FIG-081022-7

Publication

Krens et al., 2008 - ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis

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Fig. 4

Quantitative real-time PCR confirmation of the microarray results. (A-G) qPCR was performed on seven genes that showed differential regulation of expression in response to knockdown of either ERK1, ERK2 and the standard control MO control:cdh2 (NM_131081), mycn (NM_212614), erm (NM_131205), cfos (NM_205569), mos (NM_205580), snai1a (NM_131066) and vegf (NM_131408), correlated to the β-actin housekeeping gene. A comparison of the fold changes in expression of these genes, detected by qPRC assay and microarray, are listed in a table (H). ⇑ = induction of expression, ⇓ = repression of expression, compared to the standard control MO (A-C, red line).

Expression Data

Genes:	cdh2 etv5b fosab mos mycn snai1a vegfaa
Fish:	WT WT + MO1-mapk1 WT + MO3-mapk3
Knockdown Reagents:	MO1-mapk1 MO3-mapk3
Anatomical Term:	whole organism
Stage:	30%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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