Distribution of neuromasts on the caudal fins of medaka and zebrafish. A-C: Adult phenotypes visualized by DiAsp staining. The whole body with representative drawings (A,C) and the caudal fins (B,D) are shown for medaka (A,B) and zebrafish (C,D). Higher magnifications of the boxed regions are shown in insets. Neuromasts on the opposite (right) side of the caudal fin are detected as weak signals (arrows). Lateral views; anterior is to the left. fr, fin ray. E-G,I: Embryonic phenotypes. The hatched fry of medaka (E) and zebrafish (G) are stained with DiAsp. In zebrafish Isl1-GFP line, green fluorescent protein (GFP) expression is also detected in hindbrain (indicated as hb) and neural tube (nt). Insets show ventral views of the prospective caudal fin region (brackets) of the same embryos. Arrowheads indicate the terminal neuromasts. Arrows indicate neuromasts on the opposite side of the embryos. Position of the terminal neuromast is quantified in medaka (F) and in zebrafish (I, in case of three neuromasts). Positions are shown as distances from tip of the notochord (drawings in F and I). H,J: Variability in number and position of terminal neuromasts in zebrafish. H: There was no correlation in number of terminal neuromasts between the left and right sides of the body. J: Ratio of neuromasts that are located symmetrically (difference in position is less than 10 μm in length between both sides of each embryos) is shown in case of three neuromasts (T1-3, correspond to that of a drawing in I). Scale bars = 500 μm in B,D,E,G.
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