ZFIN ID: ZDB-FIG-080702-4
Cau et al., 2008 - Notch resolves mixed neural identities in the zebrafish epiphysis. Development (Cambridge, England)   135(14):2391-2401 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Long-pec

Fig. 4 Modification of neuronal subtype identity in mib and DAPT-treated embryos. (A,B) Expression of GFP (green) and Islet1 (purple) in wild-type (WT) and mib;Tg(HuC:GFP) transgenic embryos at 48 hours. As Tg(HuC:GFP) labels other structures close to the epiphysis and as the epithalamus of mib embryos is highly disorganized, Islet1 serves to identify epiphysial neurons. (C,D) Expression of GFP (green) in Tg(AANAT2:GFP) transgenic embryos shown as confocal sections with Islet1 (purple). In wild-type embryos, Tg(AANAT2:GFP)+ photoreceptors are arranged in two mirror-imaged rows with the outer segments of the cells located at the midline (white line), this stereotyped organization is lost in mib embryos. Scale bars: 18.75 μm. (E,F) Average numbers of GFP+ cells (green) in Tg(HuC:GFP) (E) or in Tg(AANAT2:GFP) embryos (F) in the epiphysis of wild-type, mib or DAPT-treated embryos at 48 hours. Anterior is upwards. Error bars represent the standard deviation *P<0.05; ***P<0.0005 using a t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg standard conditions Long-pec epiphysis IFL
mib1ta52b/ta52b; knu3Tg standard conditions Long-pec epiphysis IFL
mib1ta52b/ta52b; y8Tg standard conditions Long-pec epiphysis IFL
y8Tg standard conditions Long-pec epiphysis IFL
isl1a Ab2-isl knu3Tg standard conditions Long-pec epiphysis neuron IHC
Ab2-isl mib1ta52b/ta52b; knu3Tg standard conditions Long-pec epiphysis neuron IHC
Ab2-isl mib1ta52b/ta52b; y8Tg standard conditions Long-pec epiphysis neuron IHC
Ab2-isl y8Tg standard conditions Long-pec epiphysis neuron IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development