ZFIN ID: ZDB-FIG-080417-29
Kotani et al., 2008 - misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance. Developmental Biology   316(2):383-396 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Sphere

Fig. 9 The mys-deficient cells decreased lamellipodia formation on Fibronectin. (A, B) The morphology of cells dissociated from 4 hpf embryos and incubated on Fibronectin for overnight. (A) Cells dissociated from wild type embryos. (B) Cells dissociated from the ATG-MO injected embryos. The embryos were injected with rhodamine-dextran (red). Lamellipodia formation is impaired in the ATG-MO injected cells. (C–H) Analysis of FAK activation. Wild type cells (C, F) and the ATG-MO injected cells (D, G) incubated on Fibronectin for overnight. (E, H) Wild type cells immediately after placed on Fibronectin. (C, D, E) Images of rhodamine-dextran. (F, G, H) Immunostaining using the anti-phosphorylated FAK antibody. FAK is activated in the ATG-MO injected cells. Scale bars show 20 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-prrc1 standard conditions Sphere cell lamellipodium decreased amount, abnormal
Sphere non-membrane spanning protein tyrosine kinase activity occurrence, normal
Acknowledgments:
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Reprinted from Developmental Biology, 316(2), Kotani, T., and Kawakami, K., misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance, 383-396, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.