ZFIN ID: ZDB-FIG-080417-27
Kotani et al., 2008 - misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance. Developmental Biology   316(2):383-396 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Prim-15 to Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-25

Fig. 7 Abnormalities in the fast and slow muscle morphogenesis in the ATG-MO injected embryo. (A) The structure of the myhc3 locus and the SAGm11D insertion. T2KSAG was integrated downstream of the myhc3 gene. White boxes indicate untranslated regions and blue boxes indicate coding regions. (B) A lateral view of GFP expression in the SAGm11D embryo at 30 hpf. (C) A lateral view of a 24 hpf embryo hybridized with the myhc3 probe. (D) A confocal image of fast muscle fibers in an SAGm11D embryo at 36 hpf. (E) A confocal image of fast muscle fibers in an SAGm11D embryo injected with ATG-MO at 36 hpf. (F–I) Immunostaining using the S58 antibody that specifically detects slow muscle fibers. Confocal images of a wild type embryo (F, G) and an ATG-MO injected embryo at 32 hpf (H, I). (F, H) Lateral views of the surface area. (G, I) The same samples were rotated on 90° to generate transverse views. (J, K) Confocal images of somitic cells and adaxial cells located adjacent to the notochord. Dorsal views of a wild type embryo (J) and an ATG-MO injected embryo at 15 hpf (K). The notochord is at the bottom. Asterisks indicate the positions of the somite boundaries. The adaxial cells that elongate to span the length of the somite were formed both in wild type and the ATG-MO injected embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP nksagm011dGt standard conditions Prim-25 somite IFL
myhz1.2 nksagm011dGt standard conditions Prim-15 somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nksagm011dGt standard conditions Prim-25 trunk musculature structure, normal
nksagm011dGt + MO1-prrc1 standard conditions Prim-25 trunk musculature disorganized, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 316(2), Kotani, T., and Kawakami, K., misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance, 383-396, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.