ZFIN ID: ZDB-FIG-080417-26
Kotani et al., 2008 - misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance. Developmental Biology   316(2):383-396 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to 20-25 somites

Fig. 6 Disappearance of the somite boundaries in the mys-deficient embryo. Confocal images of wild type (A–C, G–J) and ATG-MO injected embryos (D–F, K–N). (A–F) Dorsal views of the 7th to 9th somite at 16 hpf (the 14-somite stage). (G–N) Lateral views of fast muscle cells of the 7th to 9th somite at 20 hpf (the 21-somite stage). Embryos were stained with phalloidin (A, D, G, K), anti-Fibronectin antibody (B, E, H, L), anti-phosphorylated FAK antibody (C, F, I, M) and anti-Laminin antibody (J, N). Numbers indicate the somite numbers. In the ATG-MO injected embryos, the somite boundaries disappeared at 20 hpf.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-prrc1 standard conditions 14-19 somites somite actin cytoskeleton structure, normal
14-19 somites somite laminin complex amount, normal
20-25 somites epithelial structure maintenance disrupted, abnormal
20-25 somites somite actin cytoskeleton disorganized, abnormal
20-25 somites somite laminin complex decreased amount, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 316(2), Kotani, T., and Kawakami, K., misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance, 383-396, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.