FIGURE

Fig. 2

ID
ZDB-FIG-050729-4
Publication
Xiao et al., 2005 - A GFP-based genetic screen reveals mutations that disrupt the architecture of the zebrafish retinotectal projection
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

The Brn3c:mGFP transgenic line reveals the architecture of the retinotectal projection. (A-C) Lateral view of the retinofugal projection in a 6 dpf fixed larva, whose eye has been removed (projection of a confocal z-stack). Specimen was only lightly fixed to preserve GFP label. (A) Brn3c:mGFP transgene expression. Four arborization fields, AF-6, AF-7, AF-8 and tectum (AF-10), are visible, as well as neuromasts of the lateral line. (B) Retinofugal projection, labeled with DiI following intraocular injection. (C) Merged view of the two labels shown in A and B. (D) Transverse section of 7 dpf Brn3c:mGFP transgenic larva, showing AF-7, AF-8 and the tectal layers. (E-G) Optical sections of tecta in 6 dpf live larvae (dorsal view; anterior is upwards, midline is towards the right). (E) Shh:GFP labels all four retinorecipient layers in the tectum. (F) Brn3c:mGFP labels the SO and SFGS. (G) Double-labeling in vivo with DiI and Brn3c:mGFP, showing the absence of GFP in one of the three SFGS sublaminae. For technical reasons, live DiI staining is always incomplete. As a result, some GFP-only (green) fibers are seen in the SFGS. (H) Summary of the Brn3c:mGFP labeling pattern. RGCs project to ten AFs. The largest AF, the tectum, has four layers. Only the green areas receive significant Brn3c:mGFP input. Red areas are devoid of GFP label. Yellow area (SO) is innervated mainly by non-GFP fibers, but also receives weak Brn3:mGFP innervation. AF, arborization field; SO, stratum opticum; SFGS, stratum fibrosum et griseum superficiale; SGC, stratum griseum centrale; SAC, stratum album centrale; SPV, stratum periventriculare; asterisks, melanophores in the skin. Scale bars: 50 µm in A-D; 20 µm in E-G.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development