Publication	Data		Fish	Anatomy
Collins et al., 2019	Fig. 1		pitx2ups6/ups6	atrium cardiac myofibril disorganized, abnormal atrium cardiac myofibril irregular spatial pattern, abnormal atrium damaged, abnormal atrium fibrotic, abnormal atrium hypertrophic, abnormal atrium increased size, abnormal atrium M band morphology, abnormal atrium mitochondrion morphology, abnormal atrium necrotic, abnormal atrium Z disc morphology, abnormal cardiac ventricle decreased functionality, abnormal cardiac ventricle heart contraction decreased spatial extent of a process, abnormal heart decreased functionality, abnormal
	Fig. 2		pitx2ups6/ups6 ,   pitx2ups6/+	heart contraction arrhythmic, abnormal heart contraction decreased rate, abnormal regulation of atrial cardiac muscle cell membrane depolarization increased duration, abnormal
	Fig. 3		pitx2ups6/ups6	atrium adipor2 expression increased amount, abnormal atrium esrra expression increased amount, abnormal atrium fabp4a expression increased amount, abnormal atrium foxo1a expression decreased amount, abnormal atrium pparaa expression decreased amount, abnormal atrium ppargc1a expression decreased amount, abnormal atrium prdx5 expression decreased amount, abnormal atrium ucp1 expression decreased amount, abnormal atrium zbtb16b expression increased amount, abnormal atrium mitochondrion aggregated, abnormal
	Fig. 4		pitx2ups6/ups6 ,   pitx2ups6/ups6; s974Tg	atrium sarcomere disorganized, abnormal atrium sarcomere morphology, abnormal heart contraction arrhythmic, abnormal
	Fig. 5		pitx2ups6/ups6	heart contraction arrhythmic, abnormal
Collins et al., 2018	Fig. 1		pitx2ups6/ups6	convergent extension process quality, abnormal somite myod1 expression spatial pattern, abnormal somite anterior-posterior axis decreased width, abnormal somite medial-lateral axis increased width, abnormal somite morphology, abnormal somite shape, abnormal whole organism anterior-posterior axis decreased length, abnormal
	Fig. 2		pitx2ups6/ups6	endoderm sox17 expression spatial pattern, abnormal forerunner cell group cell migration process quality, abnormal forerunner cell group mislocalised, abnormal notochord noto expression spatial pattern, abnormal presumptive paraxial mesoderm tbx16 expression spatial pattern, abnormal presumptive ventral mesoderm eve1 expression increased distribution, abnormal shield noto expression increased distribution, abnormal
	Fig. 3		pitx2ups6/ups6	convergent extension process quality, abnormal dorsal convergence decreased process quality, abnormal mediolateral intercalation decreased process quality, abnormal notochord noto expression spatial pattern, abnormal notochord cell cuboid, abnormal notochord decreased distance prechordal plate, abnormal whole organism anterior-posterior axis decreased length, abnormal
	Fig. 4		pitx2ups6/ups6; s870Tg	cell migration involved in mesendoderm migration process quality, abnormal endoderm anterior region increased width, abnormal endodermal cell movement quality, abnormal
	Fig. 7		pitx2ups6/ups6 ,   pitx2ups6/ups6; s870Tg	endoderm extracellular matrix ab2-fn labeling decreased amount, abnormal extracellular matrix organization process quality, abnormal fibronectin fibril organization decreased process quality, abnormal whole organism cxcl12b expression decreased amount, abnormal whole organism itgb1b expression increased amount, abnormal
	Fig. 7-S3		pitx2ups6/ups6	whole organism vangl2 expression decreased amount, abnormal whole organism wdpcp expression decreased amount, abnormal whole organism wnt5b expression decreased amount, abnormal
Hendee et al., 2018	Fig. 3		pitx2mw709/mw709	anterior chamber eye decreased size, abnormal ceratohyal cartilage malformed, abnormal ceratohyal cartilage position, abnormal ceratohyal cartilage size, abnormal ethmoid cartilage morphology, abnormal ethmoid cartilage size, abnormal eye absent, abnormal eye decreased size, abnormal eye morphology, abnormal eye opacity, abnormal iris hypoplastic, abnormal iris ventral region morphology, abnormal lens elongated, abnormal Meckel's cartilage malformed, abnormal Meckel's cartilage size, abnormal optic fissure closure incomplete, abnormal palatoquadrate cartilage malformed, abnormal palatoquadrate cartilage size, abnormal ventral mandibular arch morphology, abnormal
	Fig. 4		pitx2mw709/mw709	anterior chamber eye morphology, abnormal cornea morphology, abnormal corneal endothelium morphology, abnormal corneal epithelium vacuolated, abnormal corneal stroma increased thickness, abnormal corneal stroma morphology, abnormal corneal stroma position, abnormal eye morphology, abnormal iris stroma morphology, abnormal lens capsule morphology, abnormal
	Fig. 5		pitx2mw709/mw709	annular ligament morphology, abnormal anterior chamber eye decreased size, abnormal anterior chamber eye morphology, abnormal cornea abutting lens capsule, abnormal corneal epithelium irregular thickness, abnormal corneal epithelium undulate, abnormal corneal stroma irregular thickness, abnormal corneal stroma morphology, abnormal corneal stroma undulate, abnormal iris stroma morphology, abnormal
	Fig. 6		pitx2mw709/mw709	anterior chamber eye absent, abnormal anterior segment eye cartilage tissue mislocalised, abnormal anterior segment eye integument mislocalised, abnormal corneal endothelium disorganized, abnormal corneal endothelium increased thickness, abnormal corneal endothelium morphology, abnormal corneal epithelium increased thickness, abnormal corneal epithelium morphology, abnormal corneal stroma decreased thickness, abnormal corneal stroma morphology, abnormal corneal stroma undulate, abnormal eye decreased size, abnormal eye disorganized, abnormal eye morphology, abnormal iris absent, abnormal iris decreased size, abnormal
	Fig. 7		pitx2mw709/mw709	corneal endothelium Ab9-cdh2 labeling absent, abnormal corneal epithelium Ab1-keratan sulfate labeling mislocalised, abnormal corneal epithelium Ab9-cdh2 labeling decreased amount, abnormal corneal stroma Ab1-keratan sulfate labeling spatial pattern, abnormal corneal stroma Ab3-col1a1a labeling spatial pattern, abnormal corneal stroma disorganized, abnormal corneal stroma morphology, abnormal eye cartilage tissue Ab3-col1a1a labeling mislocalised, abnormal
	Fig. 8		pitx2mw709/mw709	eye col1a1a expression decreased amount, abnormal eye col1a2 expression decreased amount, abnormal eye col5a2b expression decreased amount, abnormal eye col5a3b expression decreased amount, abnormal eye dkk2 expression decreased amount, abnormal eye tert expression decreased amount, abnormal eye wnt10a expression decreased amount, abnormal eye wnt3 expression decreased amount, abnormal eye wnt4 expression decreased amount, abnormal eye wnt6b expression decreased amount, abnormal eye wnt7aa expression decreased amount, abnormal eye wnt9b expression decreased amount, abnormal
Ji et al., 2016	Fig. 3		pitx2sny3/sny3 (AB/TU) ,   pitx2sny7/sny7 (AB/TU)	camera-type eye morphogenesis disrupted, abnormal cornea morphology, abnormal eye decreased size, abnormal iris morphology, abnormal whole organism decreased size, abnormal
	Fig. 4		pitx2sny7/sny7 (AB/TU)	anterior segment eye malformed, abnormal aqueous humor decreased volume, abnormal camera-type eye morphogenesis disrupted, abnormal cornea morphology, abnormal iris morphology, abnormal
	Fig. 5		pitx2sny7/sny7 (AB/TU)	ceratobranchial 5 cartilage lacks parts or has fewer parts of type ceratobranchial 5 primary tooth, abnormal odontogenesis disrupted, abnormal tooth 3V absent, abnormal tooth 4V incomplete structure, abnormal tooth 5V absent, abnormal
	Fig. S3		pitx2sny6/sny6 (AB/TU) ,   pitx2sny15/sny15 (AB/TU)	cornea morphology, abnormal eye decreased size, abnormal iris morphology, abnormal
	Fig. S4		pitx2sny3/sny3 (AB/TU)	female organism absent, abnormal female sex determination disrupted, abnormal
	Fig. S5		pitx2sny6/sny6 (AB/TU)	anterior segment eye malformed, abnormal aqueous humor decreased volume, abnormal camera-type eye morphogenesis disrupted, abnormal ceratobranchial 5 cartilage lacks parts or has fewer parts of type ceratobranchial 5 primary tooth, abnormal cornea morphology, abnormal iris morphology, abnormal odontogenesis disrupted, abnormal tooth 3V absent, abnormal tooth 4V incomplete structure, abnormal tooth 5V absent, abnormal
	text only		pitx2sny15/sny15 (AB/TU) ,   pitx2sny7/sny7 (AB/TU)	ceratobranchial 5 cartilage lacks parts or has fewer parts of type ceratobranchial 5 primary tooth, abnormal whole organism viability, abnormal
Garric et al., 2014	Fig. 1		WT + MO5-pitx2 ,   WT + MO9-pitx2	habenula determination of left/right symmetry process quality, abnormal habenula development process quality, abnormal
	Fig. 2		zf15Tg + MO5-pitx2 ,   WT + MO5-pitx2 ,   WT + MO9-pitx2 ,   zf15Tg + MO9-pitx2	epithalamus development process quality, abnormal parapineal organ hyperplastic, abnormal pineal complex molecular quality, abnormal
	Fig. 4		WT + MO5-pitx2	epithalamus development process quality, abnormal parapineal organ hyperplastic, abnormal
	Fig. 5		WT + MO5-pitx2	habenula determination of left/right symmetry process quality, abnormal habenula development process quality, abnormal
Bohnsack et al., 2012	Fig. 1		WT + MO6-pitx2	cornea increased thickness, abnormal corneal endothelium absent, abnormal corneal epithelium disorganized, abnormal corneal epithelium scalloped, abnormal eye anterior-posterior axis decreased length, abnormal eye dorsal-ventral axis decreased length, abnormal mandibular arch skeleton malformed, abnormal mandibular muscle morphology, abnormal medial rectus increased thickness, abnormal medial rectus myofibril disorganized, abnormal photoreceptor outer segment layer absent, abnormal retinal inner nuclear layer disorganized, abnormal
Liu et al., 2012	Fig. 1		WT + MO4-pitx2	eye decreased size, abnormal head decreased size, abnormal head malformed, abnormal mandibular arch skeleton morphology, abnormal pericardium edematous, abnormal trunk decreased length, abnormal trunk kinked, abnormal whole organism viability, abnormal
	Fig. 2		WT + MO4-pitx2	anterior segment eye increased accumulation cell, abnormal anterior segment eye structure, abnormal basibranchial hypoplastic, abnormal basihyal cartilage hypoplastic, abnormal ceratobranchial cartilage hypoplastic, abnormal ceratohyal cartilage position, abnormal eye decreased size, abnormal eye shape, abnormal Meckel's cartilage hypoplastic, abnormal Meckel's cartilage position, abnormal Meckel's cartilage shape, abnormal mouth open, abnormal palatoquadrate cartilage hypoplastic, abnormal palatoquadrate cartilage shape, abnormal pharyngeal arch 2 skeleton mislocalised ventrally, abnormal ventral mandibular arch mislocalised ventrally, abnormal
	Fig. 3		WT + MO4-pitx2	anterior segment eye cell disorganized, abnormal anterior segment eye increased accumulation cell, abnormal hyaloid vessel morphology, abnormal oral cavity malformed, abnormal pharyngeal arch malformed, abnormal
	Fig. 5		y1Tg + MO4-pitx2 ,   WT + MO4-pitx2	anterior segment eye structure, abnormal camera-type eye morphogenesis process quality, abnormal cornea morphology, abnormal hyaloid vessel disorganized, abnormal iris morphology, abnormal lens decreased size, abnormal ocular blood vessel has extra parts of type hyaloid vessel, abnormal oral cavity malformed, abnormal pharyngeal arch malformed, abnormal retina shape, abnormal
Lupo et al., 2011	Fig. 6		WT + MO1-pitx2	closure of optic fissure arrested, abnormal optic fissure closure incomplete, abnormal