PUBLICATION

Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination

Authors
Okigawa, S., Mizoguchi, T., Okano, M., Tanaka, H., Isoda, M., Jiang, Y.J., Suster, M., Higashijima, S.I., Kawakami, K., Itoh, M.
ID
ZDB-PUB-140513-80
Date
2014
Source
Developmental Biology   391(2): 196-206 (Journal)
Registered Authors
Higashijima, Shin-ichi, Itoh, Motoyuki, Jiang, Yun-Jin, Kawakami, Koichi, Mizoguchi, Takamasa, Suster, Maximiliano
Keywords
Notch signaling, V2 neuron, Zebrafish
MeSH Terms
  • Signal Transduction/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Homeodomain Proteins/metabolism
  • Spinal Cord/cytology
  • Spinal Cord/embryology*
  • Animals
  • Receptors, Notch/genetics*
  • Receptors, Notch/metabolism
  • Gene Knockout Techniques/veterinary
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/metabolism
  • Cell Proliferation
  • Ubiquitin-Protein Ligases/metabolism
  • Morpholinos/genetics
  • Interneurons/cytology*
  • Neurogenesis/genetics*
  • Gene Expression Regulation, Developmental
  • Zebrafish/embryology*
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Receptor, Notch1/metabolism
  • Neural Stem Cells/cytology*
  • Neural Stem Cells/metabolism
(all 26)
PubMed
24768892 Full text @ Dev. Biol.
Abstract
The broad diversity of neurons is vital to neuronal functions. During vertebrate development, the spinal cord is a site of sensory and motor tasks coordinated by interneurons and the ongoing neurogenesis. In the spinal cord, V2-interneuron (V2-IN) progenitors (p2) develop into excitatory V2a-INs and inhibitory V2b-INs. The balance of these two types of interneurons requires precise control in the number and timing of their production. Here, using zebrafish embryos with altered Notch signaling, we show that different combinations of Notch ligands and receptors regulate two functions: the maintenance of p2 progenitor cells and the V2a/V2b cell fate decision in V2-IN development. Two ligands, DeltaA and DeltaD, and three receptors, Notch1a, Notch1b, and Notch3 redundantly contribute to p2 progenitor maintenance. On the other hand, DeltaA, DeltaC, and Notch1a mainly contribute to the V2a/V2b cell fate determination. A ubiquitin ligase Mib, which activates Notch ligands, acts in both functions through its activation of DeltaA, DeltaC, and DeltaD. Moreover, p2 progenitor maintenance and V2a/V2b fate determination are not distinct temporal processes, but occur within the same time frame during development. In conclusion, V2-IN cell progenitor proliferation and V2a/V2b cell fate determination involve signaling through different sets of Notch ligand-receptor combinations that occur concurrently during development in zebrafish.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
nkhspGFFDMC72AEtTransgenic Insertion
nkuasgfp1aTgTransgenic Insertion
    nns5TgTransgenic Insertion
      ta52b
        Point Mutation
        th35b
          Point Mutation
          tit446
            Point Mutation
            tm98
              Point Mutation
              tr233
                Point Mutation
                1 - 8 of 8
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Fish
                Antibodies
                Orthology
                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                GFPEFGGFP
                1 - 2 of 2
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                Mapping