PUBLICATION

Cell-Fibronectin Interactions Propel Vertebrate Trunk Elongation via Tissue Mechanics

Authors
Dray, N., Lawton, A., Nandi, A., Jülich, D., Emonet, T., and Holley, S.A.
ID
ZDB-PUB-130710-101
Date
2013
Source
Current biology : CB   23(14): 1335-41 (Journal)
Registered Authors
Dray, Nicolas, Holley, Scott, Jülich, Dörthe
Keywords
none
MeSH Terms
  • Cell Movement
  • Extracellular Matrix/metabolism
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Tail/embryology
  • Tail/metabolism
  • Integrin alphaV/genetics
  • Integrin alphaV/metabolism
  • Animals
  • Cell Adhesion
  • Integrin alpha5/genetics
  • Integrin alpha5/metabolism
  • Fibronectins/genetics*
  • Fibronectins/metabolism
(all 19)
PubMed
23810535 Full text @ Curr. Biol.
Abstract

During embryonic development and tissue homeostasis, cells produce and remodel the extracellular matrix (ECM). The ECM maintains tissue integrity and can serve as a substrate for cell migration. Integrin α5 (Itgα5) and αV (ItgαV) are the α subunits of the integrins most responsible for both cell adhesion to the ECM protein fibronectin (FN) and FN matrix fibrillogenesis [1 and 2]. We perform a systems-level analysis of cell motion in the zebrafish tail bud during trunk elongation in the presence and absence of normal cell-FN interactions. Itgα5 and ItgαV have well-described roles in cell migration in vitro. However, we find that concomitant loss of itgα5 and itgαV leads to a trunk elongation defect without substantive alteration of cell migration. Tissue-specific transgenic rescue experiments suggest that the FN matrix on the surface of the paraxial mesoderm is required for body elongation via its role in defining tissue mechanics and intertissue adhesion.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
tbfe1
    Point Mutation
    tbfe2
      Point Mutation
      ya10TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        itga5MO1-itga5MRPHLNO
        itgavMO5-itgavMRPHLNO
        1 - 2 of 2
        Show
        Fish
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        RFPEFGRFP
        1 - 1 of 1
        Show
        Mapping
        No data available