FIGURE

Fig. 5

ID

ZDB-FIG-260702-129

Publication

Breuer et al., 2026 - Chd7 regulates lipid metabolism and swim bladder inflation in zebrafish

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Fig. 5

PPARγ in swim bladder development. A: Representative morphology and ORO staining of sibling 5dpf chd7^+/+ (vehicle treated) and B: BADGE treated larvae with close up of caudal vein region C: ORO staining showing neutral lipid droplet accumulation in the eye vasculature and swim bladder (arrows) of 5dpf chd7^+/+(vehicle treated), chd7^−/− mutants and BADGE treated chd7^+/+ larvae. D: Representative morphology and ORO staining of 5dpf chd7^+/+ and (E) chd7^−/− mutants larvae treated with RGZ, with a close-up of the caudal vein region. A–E: representative images for 3 independent experiments (n = 5larvae per condition and genotype). F. RT-qPCR of key swim bladder lipid metabolism markers fabp7b, elov1a, anxa5b and cebpa in chd7^+/+ (vehicle-treated), early and late onset BADGE-treated and RGZ-treated larvae. RT-qPCR were performed with sibling wildtype (chd7^+/+) larvae of 3 separate clutches (N) (N = 3 from 15 pooled fish per sample/genotype; significance: ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; Error bars represent SD).

Expression Data

Genes:	anxa5b cebpa elovl1a fabp7b
Fish:	WT
Conditions:	chemical treatment by environment: Bisphenol A diglycidyl ether chemical treatment by environment: rosiglitazone
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT chd7^{zf4383/zf4383}
Conditions:	chemical treatment by environment: Bisphenol A diglycidyl ether chemical treatment by environment: rosiglitazone
Observed In:	ocular blood vessel lipid droplet swim bladder lipid swim bladder lipid droplet swim bladder lipid transport vasculature lipid whole organism whole organism lipid yolk lipid yolk lipid transport
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Lipid Res.