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Kif15 deficiency leads to impaired development of dendritic spines in PFC and hippocampus of mice. A: Experimental design. WB: Western Blot; IF: immunofluorescence staining. B-D: Representative images of neuronal dendrites by Golgi staining in layer2/3 neurons of PFC at 3,6 and 11 week-old Kif15+/+ and Kif15-/- mice, Scale bar = 5μm. H-J: Representative images of neuronal dendrites by Golgi staining in CA1 of hippocampus at 3,6 and 11 week-old Kif15+/+ and Kif15-/- mice, Scale bar = 5μm. B, C, E and F: Decreased densities of total spine and mushroom-like spines in the PFC neurons from Kif15-/- in 3, 6 week-old mice, compared with Kif15+/+ mice, n = 15 dendrites of neurons from 3 mice in each group. H, I, K and L: Decreased densities of total spine and mushroom-like spines in the CA1 of hippocampus from Kif15-/- in 3, 6 week-old mice, compared with Kif15+/+ mice, n = 15 dendrites of neurons from 3 mice in each group. D and G: Similar total dendritic spine density between Kif15+/+ and Kif15-/- in 11 week-old mice expect for filopodia in PFC. J and M: Similar total dendritic spine density between Kif15+/+ and Kif15-/- in 11 week-old mice expect for mushroom like spine in hippocampus. The densities of different types of dendritic spines in PFC neurons from different weekly mice were quantified, n = 15 dendrites of neurons from 3 mice in each group. N: Diagram to show the whole-cell recording of pyramidal neurons in layer 2/3 of PFC from Kif15+/+ and Kif15-/- mice. (O-Q): Representative mEPSCs traces of neurons in PFC from 3, 6 and 11 week-old mice. R: Decreased mEPSCs frequency and similar amplitude of neurons in 3 week-old, n = 20 neurons from 6 Kif15+/+ mice, n = 14 neurons from 5 Kif15-/- mice. S: Decreased frequency but increased amplitude of mEPSCs in PFC neurons in 6 week-old Kif15-/- mice, compared with Kif15+/+ mice, n = 12 neurons from 5 Kif15+/+ mice, n = 17 neurons from 5 Kif15-/- mice. T: Similar frequency and decreased amplitude of mEPSCs in PFC Kif15-/- mice at 11 week old, n = 23 neurons from 4 Kif15+/+ mice, n = 23 neurons from 4 Kif15-/- mice. All the data are presented as mean±SD, *P < 0.05, **P < 0.01,two-tailed student’s t test between two groups, Multiple t test performed in different morphology spine group.
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