PUBLICATION
Deficiency of Kif15 impairing synaptic development leads to mood disorder in mice
- Authors
- He, X., Zhang, W., Chen, X., Dong, Z., Wei, C., Wu, T., Kong, D., Kong, R., Wu, R., Liu, Y., Liu, M.
- ID
- ZDB-PUB-250903-1
- Date
- 2025
- Source
- PLoS Genetics 21: e1011839e1011839 (Journal)
- Registered Authors
- Dong, Zhangji
- Keywords
- none
- MeSH Terms
-
- Dendritic Spines/genetics
- Dendritic Spines/metabolism
- Dendritic Spines/pathology
- Humans
- Mice, Knockout
- Receptors, N-Methyl-D-Aspartate/genetics
- Receptors, N-Methyl-D-Aspartate/metabolism
- Animals
- Membrane Proteins/genetics
- Membrane Proteins/metabolism
- Neurons/metabolism
- Mood Disorders*/genetics
- Mood Disorders*/metabolism
- Mood Disorders*/pathology
- Disease Models, Animal
- Synapses*/genetics
- Synapses*/metabolism
- Synapses*/pathology
- Disks Large Homolog 4 Protein/genetics
- Disks Large Homolog 4 Protein/metabolism
- Bipolar Disorder*/genetics
- Male
- Kinesins*/deficiency
- Kinesins*/genetics
- Mice
- PubMed
- 40892874 Full text @ PLoS Genet.
Citation
He, X., Zhang, W., Chen, X., Dong, Z., Wei, C., Wu, T., Kong, D., Kong, R., Wu, R., Liu, Y., Liu, M. (2025) Deficiency of Kif15 impairing synaptic development leads to mood disorder in mice. PLoS Genetics. 21:e1011839e1011839.
Abstract
The harmony of neuronal excitation and inhibition is essential for precise neuronal circuitry in the developmental brain, and thus affects the human emotion. Abnormalities of synaptic morphology directly affect neuronal function and contribute to a variety of psychiatric disorders. Previous studies have shown that Kif15 (Kinesin-12), a microtubule-associated motor protein, affects neurite growth, navigation, and branching during neuronal development, revealing the potential of Kif15 to influence neuronal dendritic morphology. A GWAS study in a European population showed that there were variants in both exons and introns of the KIF15 gene on chromosome 3 in patients with depression. Therefore, we generated Kif15-/- mice using CRISPR/Cas9 technology. In this study, we found that Kif15-/- mice have exhibited significant impacts on dendritic morphology and function, which contributes to mood disorders. Compared with Kif15 wild-type mice, adolescent Kif15-/- mice showed a significant decrease in the excitatory postsynaptic scaffolding protein PSD95 and NMDA receptors, as well as a reduction in the total density of dendritic spines and the density of mushroom spines, and a decrease in the frequency of mEPSCs. Meanwhile, the inhibitory postsynaptic scaffold protein Gephyrin and GABRB1 significantly upregulated. However, the adult Kif15-/- mice simultaneously exhibited an obvious manic behavior and their PSD95 expression increased rapidly, even more than that of the Kif15 wild-type mice. Meanwhile, overexpression of Kif15 in kif15-/- zebrafish rescued their depressive behavior. In terms of molecular mechanism, we showed that KIF15 interacted with PSD95 protein using both endogenous and exogenous Co-IP assays. Furthermore, we found that PSD95 in Kif15-/- mice was distributed around neuronal nuclei, in contrast to PSD95 localized close to the cell membrane in Kif15 wild-type mice. In conclusion, our study has identified a microtubule-associated molecular motor, KIF15, that plays a novel role in bipolar disorder through its contributions to spine morphology and function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping