FIGURE

Fig. 4

ID

ZDB-FIG-250814-50

Publication

Kelu et al., 2025 - Muscle peripheral circadian clock drives nocturnal protein degradation via raised Ror/Rev-erb balance and prevents premature sarcopenia

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Fig. 4

Ulk1-dependent autophagy activation at night limits nocturnal muscle growth. (A) The autophagy pathway highlighting relevant proteins and inhibitors (red). (B) Circadian mRNA levels of ulk1a and ulk1b in 3xLD entrained whole larvae of control and mΔCLK siblings under free-run analyzed by RT-qPCR (SI Appendix, Fig. S1C). Statistics from JTK_Cycle. (C) Circadian oscillation in Ulk1Ser757 phosphorylation in larvae under free-run between 3 and 4 dpf assessed by western blotting. Statistics are two-way ANOVA (Lay/Time) with Bonferroni’s post hoc test. (D) Circadian variations in autophagic degradation of p62 and LC3-II in larvae under free-run treated with DMSO, bafilomycin A1 (Baf A1) alone, or both Baf A1 and MRT68921 (MRT) during either CT0-12 or CT12-24 and analyzed by western blotting. Statistics are two-way ANOVA (Lay/Treatment) with Bonferroni’s post hoc test. (E and F) Circadian myotome growth after autophagy inhibition in entrained active or inactive larvae treated under free-run between 3 and 4 dpf with DMSO, MRT68921 (E), or bafilomycin A1 (F). Statistics are two-way ANOVA (Time/Treatment) with Bonferroni’s post hoc test.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA