Fig. 4

Contribution of de novo hepatocytes to regeneration following partial hepatectomy (PHx).

A Schematic showing the potential modes of regeneration after PHx and the associated fluorescent labeling of hepatocytes. B Experimental strategy for 4-OHT-pulse labeling and PHx in the Tg(fabp10a:CellCousin) background. C Representative confocal images of the left lobe from live imaging of the Tg(fabp10a:CellCousin) animals that were uninjured or subjected to PHx. D Min-to-max boxplot showing quantification of the normalized number of mTagBFP2+ cells in uninjured (n = 29 animals) and PHx animals (n = 30 animals) at 4 dpi (Two-tailed Mann–Whitney test). Each dot represents one animal; mean ± SEM is indicated above each box. Scale bar: 200 μm (B). E Barplot with Mean ± SEM showing quantification of the percentage of mTagBFP2+ hepatocytes using FACS in uninjured (n = 4 biological replicates) and 4 dpi (n = 3 biological replicates) animals (Two-tailed Welch’s t-test). Each dot represents one biological replicate; mean ± SEM is indicated above each box.